Lipoamide dehydrogenase, which is a common component of alpha-keto acid dehydrogenase complexes, has been highly purified from yeast (Saccharomyces cerevisiae) to reveal its structure at higher resolution. New crystals obtained by a desalting method exhibited diffraction beyond 2.5 A resolution. The cell dimensions are a = 97.1, b = 158.7, and c = 67.9 A, and the space group is P2(1)2(1)2(1). T...

The utility of a laboratory diagnostic approach was investigated for a systematic evaluation of the impact of operating conditions, membrane type, and antiscalant effectiveness on membrane mineral salt scale formation. The study focused on low-pressure reverse osmosis (RO) membrane desalting of brackish water with high potential for scaling by calcium sulfate dihydrate (gypsum). Gypsum scale fo...

A simulated moving-bed adsorber (SMBA)consisting of only three or four columns packed with gel chromatographic materials was applied for the continuous desalting of bovine serum albumin (BSA) from ammoniumsulfate. It was shownexperimentally that the solutes could be continuously recovered in different streams at concentrations of over 50% of their feed concentrations. The recovery of BSAwas in ...

ABSTRACT The lethal threats to human health from heavy metals are associated with untreated ground water. We developed a high throughput single step heavy metal purification process based on Ion Concentration Polarization mechanism. Instead of microfluidic channel networks, the plastic prototype made of plastic meshes and Nafion nanoporous material was built so that the manufacturability and co...

We present a microfluidic device with integrated temperature sensors and heaters for single nucleotide polymorphism (SNP) detection. The allele specific primer is immediately annealed next to the polymorphic site, and extended by a single base. The extension product is then purified by solid phase capture, and subsequently released via chemical cleavage. After desalting, the released product is...

The aim of this work was to isolate and purify bromelain extracted from the pineapple peel by ammonium sulfate precipitation (40-80%), followed by desalting and freeze-drying with a 75% activity recovery and 2.2 fold increased specific activity. Ion exchange chromatography on DEAE-Sepharose was able to separate the polysaccharides from the enzyme, which was recovered in the elution step, mainta...

Amino acids have appreciable vapor pressures above 150 degrees C and will sublime under partial vacuum at elevated temperatures without any racemization or decomposition. The recoveries of several amino acids including aspartic acid, serine, glycine, alanine, alpha-aminoisobutyric acid, and valine were optimized by varying the temperature and duration of sublimation. Sublimation has been shown ...