purpose: to report the clinico-pathological and ultrastructural features of a case of meesmann corneal dystrophy (mcd). case report: a 10-year-old boy presented with decreased vision in both eyes since early childhood. slitlamp biomicroscopy disclosed diffuse intraepithelial microcystic changes in the cornea but other layers appeared unremarkable. high pathologic myopia and amblyopia were other...

PURPOSE To update the 2008 International Classification of Corneal Dystrophies (IC3D) incorporating new clinical, histopathologic, and genetic information. METHODS The IC3D reviewed worldwide peer-reviewed articles for new information on corneal dystrophies published between 2008 and 2014. Using this information, corneal dystrophy templates and anatomic classification were updated. New clinic...

PURPOSE Previous studies have indicated that muscarinic acetylcholine receptors (mAChR) may be present in an unexpected, unique location and play a singular role in cellular growth regulation of rabbit corneal epithelium that may be of general physiologic significance if found in other cells. The purpose of this study was to examine rabbit corneas and corneal cells in culture to determine mAChR...

Normal vision depends, in part, on the combined refractive powers of the cornea and crystalline lens to permit adequate focusing of light onto the retina. Such refractive function requires that the cornea remain transparent, a requirement that is met provided that corneal hydration, i.e., deturgescence, is maintained within specific physiological limits. Maintenance of corneal deturgescence is ...

PURPOSE To identify the role of Notch signaling in the human corneal epithelium. METHODS Localization of Notch1, Notch2, Delta1, and Jagged1 in the human corneal epithelium was observed with the use of indirect immunofluorescence microscopy. Gene and protein expression of Notch receptors and ligands in human corneal epithelial cells was determined by RT-PCR and Western blot analysis, respecti...

The authors compared cytoskeletal elements of the in situ human trabecular-meshwork cell with in situ human corneal cells using indirect immunofluorescence staining for tubulin and intermediate filaments (vimentin, cytokeratin, and desmin) and NBD-phallacidin staining for f-actin using both fixed frozen and unfixed frozen sections from postmortem eyes. Both f-actin and tubulin were found throug...

PURPOSE Although cryotherapy has long been used to eradicate corneal lesions, there have been no reports of adverse effects of cryotherapy on human corneas. We performed this study to evaluate and characterize ultrastructural damage to the human cornea following the transcorneal freezing-and-thawing procedure. METHODS Seven human donor corneas were randomly divided into three groups. 1, 2, an...

An experimental study examined the effect of basic fibroblast growth factor (bFGF) on the healing of corneal epithelial wounds in dogs. A corneal wound was made on one eye from each of 40 dogs with a corneal trephine (6 mm diameter). Four concentrations of bFGF (0, 0.1, 0.5, and 1.0 μg/ml) were applied to the affected eyes three times daily. Fluorescein staining was used to assess the closure o...

PURPOSE We report a case of Terrien's marginal degeneration (TMD) with a unilaterally typical narrow band of peripheral corneal stroma thinning, accompanied by the presence of an unusual network of opacities diffusing throughout the anterior stroma layers. CASE REPORT A 43-year-old woman presented with superior nasal peripheral corneal thinning and an unusual network of polygonal stromal opac...