conclusions immediate diagnosis based on careful history and physical examination are essential for management of botulism. people should be notified about proper food handling and preparation of traditional homemade foods. patients and methods during an outbreak, 10 members of a family referred to the hospital because of food poisoning. all patients had a history of consumption of doogh, a tra...

clostridium diffcile is part of the normal intestinal flora in a small proportion of healthy persons. when exposed to antibiotics, the normal enteric flora is altered, permitting the overgrowth of these relatively resistant organisms. proliferation of the organisms with localized production of their toxins leads to disease (pseudomembran colitis or antibiotic associated diarrhea). in this study...

An amplified fragment length polymorphism (AFLP) method was applied to 129 strains representing 24 different Clostridium species, with special emphasis on pathogenic clostridia of medical or veterinary interest, to assess the potential of AFLP for identification of clostridia. In addition, the ability of the same AFLP protocol to type clostridia at the strain level was assessed by focusing on C...

Late blowing defect (LBD) is a major cause of spoilage in cheeses, caused by the growth of Clostridium spp. in the cheese matrix. We investigated the application of CTP1L, a bacteriophage endolysin active against Clostridium tyrobutyricum, and its enzymatically active and cell wall-binding domains (EAD and CBD) attached to green fluorescent protein (GFP) to detect dairy-related Clostridium spec...

Small subunit rDNA sequences were determined for 20 species of the genera Acetogenium, Clostridium, Thermoanaerobacter, Thermoanaerobacterium, Thermoanaerobium, and Thermobacteroides, 3 non-validly described species, and 5 isolates of anaerobic thermophilic bacteria, providing a basis for a phylogenetic analysis of these organisms. Several species contain a version of the molecule significantly...

BACKGROUND The human gut microbiome is important for maintaining the health status of the host. Clostridia are key members of the human gut microbiome, carrying out several important functions in the gut environment. Hence understanding the role of different Clostridium species isolated from human gut is essential. The present study was aimed at investigating the role of novel Clostridium sp. i...

Clostridium difficile has been implicated in the relapse of ulcerative colitis. Controversy exists over this role and its relationship to sulphasalazine exposure. Sixty two of 77 patients with a documented relapse of ulcerative colitis were investigated for the presence of Clostridium difficile, or its toxin, prior to hospitalization. There was a low incidence of detection which was related to ...

Results A total of 47 critically ill patients (32 men, mean age 66 ± 9 years, mean SOFA-score on admission 9 ± 3) with Clostridium non-difficile infection were included in this study. The most common pathogens were Clostridium innocum (n = 23), Clostridium perfringens (n = 11), Clostridium tertium (n = 6) and others (n = 7). Pathogens were detected in 62% intra-abdominal, in 29% in blood cultur...

Detection and Frequency of Enterotoxin (cpa,cpe) Genes Clostridium perfringens Isolated from Dehydrated Vegetables by PCR