Raw hospital wastewater is a source of excessive heavy metals and pharmaceutical pollutants. In water-stressed countries such as Pakistan, the practice unsafe reuse by local farmers for crop irrigation major concern. our previous work, we developed low-cost bacterial consortium treatment method. Here, in two-part study, first aimed to find what physico-chemical parameters were most important di...

The objective of this investigation was to assess the direct and residual effect graded levels boron (B) on growth, yield quality parameters crops in cauliflower-cowpea-okra cropping sequence. For experimentation, cauliflower grown as a test crop B fertilization while cowpea okra were succeeding fertilization. study revealed an incremental trend plant physiological corresponding escalating leve...

A rapid, simple and an efficient method for the determination of indoxacarb in cauliflower and soil samples was developed and validated using QuEChERS technique (Quick, Easy, Cheap, Effective, Rugged and Safe). Recoveries at four different spiking concentrations of 0.01, 0.05, 0.1 and 0.2 mg·kg ranged from 87% to 96% were achieved with good repeatability and RSD of 1% 6%. The average initial de...

Telomerase activity is developmentally regulated in mammals. Here we examine telomerase activity in plants, whose development differs in fundamental ways from that of animals. Using a modified version of the telomere repeat amplification protocol (TRAP) assay, we detected an activity in extracts from carrots, cauliflower, soybean, Arabidopsis, and rice with all the characteristics expected for ...

The gene encoding a XET (xyloglucan endotransglycosylase) from cauliflower ( Brassica oleracea var. botrytis ) florets has been cloned and sequenced. Sequence analysis indicated a high degree of similarity to other XET enzymes belonging to glycosyl hydrolase family 16 (GH16). In addition to the conserved GH16 catalytic sequence motif EIDFE, there exists one potential N-linked glycosylation site...

The Swede midge, Contarinia nasturtii Kieffer (Diptera: Cecidomyiidae), a common insect pest in Europe, is a newly invasive pest in North America that constitutes a major threat to crucifer vegetable and field crops. Chemical control of Swede midge with synthetic insecticides under laboratory conditions indicated that insecticides generally could provide very effective control; however, insecti...

Pieris brassicae L. is a serious pest of cultivated crucifers in several parts of the world. Larvae of P. brassicae also feed prolifically on garden nasturtium (Tropaeolum majus L., of the family Tropaeolaceae). Proteolytic digestion was studied in larvae feeding on multiple hosts. Fourth instars were collected from cauliflower fields before transfer onto detached, aerial tissues of selected ho...

9 Investigation was made to confirm the stability of drought and salt stress tolerance in 10 cauliflower (Brassica oleracea var.botrytis) mutants after regeneration and micropropagation. 11 The N-nitroso-N-ethyleurea (NEU) and N-nitroso-N-methylurea (NMU) induced mutants of 12 cauliflower were created and screened for drought and salt stress tolerance. The highly 13 tolerant mutants were select...

We cloned a major aliphatic glucosinolate (GSL) gene, BoGSL-ELONG in Brassica oleracea, using the Arabidopsis sequence database. We based our work on an Arabidopsis candidate gene forming part of a gene family coding for isopropyl malate synthetase-like enzymes (IPMS). This gene is presumably responsible for synthesis of GSL possessing side chains consisting of four carbons (4C). The similarity...

We have investigated the influence of stress conditions such as incubation at 4 degrees C and incubation in hyperoxygen atmosphere, on plant tissues. The ubiquinone (Q) content and respiratory activity of purified mitochondria was studied. The rate of respiration of mitochondria isolated from cold-treated green bell peppers (Capsicum annuum L) exceeds that of controls, but this is not so for mi...