conclusions the findings show fluconazole -resistance in c. albicans isolates with overexpression of pir1p. results in the 52 isolates, 49 (94%) were resistant to fluconazole. overexpression of pir1 gene was detected in 47 (96%) fluconazole-resistant c. albicans isolates. patients and methods a total of 52l vaginal samples were obtained from the females with c. albicans vaginitis. the azole sus...

Hsp90 is a molecular chaperone that governs drug resistance, morphogenesis, and virulence in the leading human fungal pathogen Candida albicans. Previous work with Saccharomyces cerevisiae and C. albicans established acetylation as a novel mechanism of post-translational control of Hsp90 in fungi and implicated lysine deacetylases (KDACs) as key regulators of resistance to the most common class...

Azole resistance has been insufficiently investigated in the yeast Candida tropicalis. Here we determined the molecular mechanisms responsible for azole resistance in a clinical isolate of this pathogenic yeast. Antifungal susceptibility testing performed by a disk diffusion method showed resistance or markedly decreased susceptibility to azoles, which was confirmed by determination of MICs. Co...

Candida albicans is the most prevalent cause of fungemia worldwide. Its ability to develop resistance in patients receiving azole antifungal therapy is well documented. In a murine model of systemic infection, we show that ibuprofen potentiates fluconazole antifungal activity against a fluconazole-resistant strain, drastically reducing the fungal burden and morbidity. The therapeutic combinatio...

Antifungal azole drugs inhibit the synthesis of ergosterol and cause the accumulation of sterols containing a 14α-methyl group, which is related to the properties of cell membrane. Due to the frequent recurrence of fungal infections and clinical long-term prophylaxis, azole resistance is increasing rapidly. In our research, Nsg2p, encoded by the ORF19.273 in Candida albicans, is found to be inv...

We describe the acquisition of flucytosine, azole, and caspofungin resistance in sequential Candida glabrata bloodstream isolates collected from a bone marrow transplant patient with clinical failure. Point mutations in C. glabrata FUR1 (CgFUR1) and CgFKS2 and overexpression of CgCDR1 and CgCDR2 were observed in resistant isolates.

ASP2397 is a new compound with a novel and as-yet-unknown target different from that of licensed antifungal agents. It has activity against Aspergillus and Candida glabrata. We compared its in vitro activity against wild-type and azole-resistant A. fumigatus and A. terreus isolates with that of amphotericin B, itraconazole, posaconazole, and voriconazole. Thirty-four isolates, including 4 wild-...

A 1.8-million-member D-octapeptide combinatorial library was constructed in which each member comprised a diversity-containing N-terminal pentapeptide and a C-terminal amidated triarginine motif. The C-terminal motif concentrated the library members at the fungal cell surface. A primary screen for inhibitors of Saccharomyces cerevisiae and Candida albicans growth, together with an in vitro seco...

fungal pathogen in humans, causes diseases varying from superficial mucosal complaints to life-threatening systemic disorders. Coincident with the increased use of antifungal drugs, the incidences of drug resistance have also increased. In recent years, the development of multidrug resistance in clinical isolates has challenged effective treatment of the infections. Specifically, the extensive ...