An extracellular alkaline protease producing B. amyloliquefaciens SP1 was isolated from apple rhizosphere having multifarious plant growth-promoting activities. B. amyloliquefaciens SP1 protease was immobilized using various concentrations of calcium alginate, agar and polyacrylamide to determine the optimum concentration for formation of the beads. Enzyme activity before immobilization (at 60 ...

The development and application of an immobilized horseradish peroxidase (HRP) on calcium alginate gel beads are reported for the successful and effective decolorization textile industrial effluent. The optimal condition for the immobilization of HRP on calcium alginate gel beads which leads to a specific activity of 15 U/g calcium alginate was identified. Upon immobilization, considering enzym...

BACKGROUND A column sorption study was carried out using calcium alginate gel beads as adsorbent for the removal of boron from aqueous solutions. The breakthrough curve was obtained as a function of pH, initial concentration of boron, feed flow rate, adsorbent mass and column diameter. The breakthrough capacity values and adsorption percentage of calcium alginate gel for boron were...

We examine the gelation of alginate undergoing ionic crosslinking upon ultraviolet (UV) irradiation using in situ dynamic rheology. Hydrogels are formed by combining alginate with calcium carbonate (CaCO3) particles and a photoacid generator (PAG). The PAG is photolyzed upon UV irradiation, resulting in the release of free calcium ions for ionic crosslinking. The viscous and elastic moduli duri...

OBJECTIVES The aim of this study was to investigate the antimicrobial activities of calcium ions and other cross-linking agents of alginate dressings, as well as their compatibility with commonly used topical antimicrobials. METHODS The antimicrobial activities of cross-linking agents and antimicrobials (five antibiotics and four antiseptics) were evaluated by the broth dilution method. The i...

The phosphate-embedded calcium alginate beads were successfully synthesized based on sodium alginate, calcium dihydrogen phosphate and sodium hydrogen carbonate. Scanning electron microscopy, Fourier transformed infrared (FTIR) spectroscopy and X-ray diffraction (XRD) were conducted to characterize the morphology and structure of the phosphate-embedded calcium alginate beads. The effects of pH ...

Protease production by Bacillus subtilis with free and immobilized cells was examined in this study. Entrapment method of immobilization was used with gelatin, polyacrylamide, calcium alginate and agar matrices and protease production was studied compared to equivalent weight of free cells. Results showed that the gelatin entrapped cells produce 10.8 U/ml; the maximum enzyme titer followed by p...

Calcium alginate (CaALG) hydrogel beads and two sets of composite beads, formed from a combination calcium alginate/propylene glycol alginate/human serum albumin (CaALG/PGA/HSA) with the quaternary ammonium salt, (3-(trimethoxysilyl)propyl)-octadecyldimethylammonium chloride (QA), (CaALG/QA), were prepared. Bovine (BSA) was condensed glutaraldehyde (GLA) to form BSA/GLA hydrogel. The correspond...

Plasters and renders used in historic monuments are vulnerable to degradation caused by salt weathering. Crystallisation inhibitors (molecules/ions that alter crystallisation) mixed into mortars have shown promising results mitigating damage inhibiting crystallisation, promoting transport the evaporating surface, modifying crystal habit. However, past research suggests easily leach out from mor...

Background: Edible films and coatings have potential benefits for food packaging, particularly meat products. These coatings prevent weight loss and texture changes during shelf life by reducing moisture loss in fresh and frozen meat products. In this study, effect of calcium alginate edible coating on microbial and chemical properties of refrigerated lamb meat over a period of  5 days was...