Complement is implicated in the pathogenesis of rheumatoid arthritis (RA); elevated levels of complement activation products have been measured in plasma, synovial fluid, and synovial tissues of patients. Complement polymorphisms are associated with RA in genome-wide association studies. Coding-region polymorphisms may directly impact protein activity; indeed, we have shown that complement poly...

A stereoselective practical synthetic route to indolmycin is described. The route is composed of the regioselective coupling of indolyl magnesium halide with a trans-epoxy ester, diastereoselective oxazolone ring formation with guanidine and amine exchange reaction with methylamine. In the coupling step, use of dichloromethane as co-solvent and conversion of the resulting hydroxy ester to the h...

A graph is C5-saturated if it has no five-cycle as a subgraph, but does contain a C5 after the addition of any new edge. Extending our previous result, we prove that the minimum number of edges in a C5-saturated graph on n vertices is sat(n, C5) = ⌈10(n − 1)/7⌉ − 1 for 11 ≤ n ≤ 14, or n = 16, 18, 20, and is ⌈10(n − 1)/7⌉ for all other n ≥ 5, and we also prove that the only C5-saturated graphs w...

in the present work, b-zsm-5 zeolite membranes were synthesized on porous tubular α-alumina supports by several subsequence in situ crystallization hydrothermal treatments. the tio2- bohmite and ɣ- alumina intermediate layers were applied to improve the lattice matching between zeolite layer and the support. the uniform membrane intermediate layers with low permeation resistance were prepared o...

Background and Importance: The development of C5 root palsy is a well-known potential complication of cervical spine surgeries for the correction of cervical spondylotic myelopathy. It typically occurs unilaterally but on extremely rare occasions it might occur as a bilateral anomaly. The value of intraoperative neurophysiologic monitoring in detection of iatrogenic acute versus delayed onset C...

The first recognized human kindred with hereditary deficiency of the fifth component of complement (C5) is described. The proband, a 20-year-old black female with systemic lupus erythematosus since age 11, lacked serum hemolytic complement activity, even during remission. C5 was undetectable in her serum by both immunodiffusion and hemolytic assays. Other complement components were normal durin...

The photoinduced hydrogen evolution reaction (HER) by decamethylruthenocene, Cp2 *RuII (Cp*=C5 Me5 ), is reported. The use of a metallocene to photoproduce hydrogen is presented as an alternative strategy to reduce protons without involving an additional photosensitizer. The mechanism was investigated by (spectro)electrochemical and spectroscopic (UV/Vis and 1 H NMR) measurements. The photoacti...

Milk performance (protein and fat content) and milk fatty acids (FA) profile of dairy cows under supplementation with two sources of concentrate, oilseeds (C, cottonseed) at two levels, low (5kg DM/cow/day) and high (7 kg DM/cow/day), and cereal grains (B, barley) at high rate (7 kg DM/cow/day), were studied in three herds (n=36) of spring calving Holstein-Friesian dairy cows (200 days in milk)...

Staphylococcus aureus secretes the SSL7 protein as part of its immune evasion strategy. The protein binds both complement C5 and IgA, yet it is unclear whether SSL7 cross-links these two proteins and, if so, what purpose this serves the pathogen. We have isolated a stable IgA-SSL7-C5 complex, and our crystal structure of the C5-SSL7 complex confirms that binding to C5 occurs exclusively through...

We recently suggested that sites of length polymorphisms in protein families (indels) might serve as useful guides for locating protein:protein interaction sites. This report describes additional site-specific mutagenesis and synthetic peptide inhibition studies aimed at testing this idea for the paralogous complement C3, C4, and C5 proteins. A series of C5 mutants was constructed by altering t...