the ph dependence study reveals that the cys 206 sulphydryl group of bovine carbonicanhydrase in the native form is not exposed. during the reaction of 2,2'-dithiobispyridine (2-dtp) with the enzyme, there was no absorbance change recorded. in the presence ofsurfactants, the ph dependence profiles of the apparent second order rate constants, kapp, forthe reaction of 2-dtp with bovine carbo...

abstract aims: bovine mastitis continues to be the most costly disease to the dairy farmers. it dominates in iran as one of the most prevalent diseases in dairy cattle among the dairy farms. mastitis treatment with antibiotics leads to the development of antibiotic resistant strains and consumer health problem. this study was performed for the first time to analyze in vitro effects of hydro-alc...

cryptosporidium is a globally distributed protozoan parasite and one of the most common causes of infection and diarrhea in humans and cattle. the aim of the present study was to determine the species of cryptosporidium among cattle with diarrhea by a nested pcr-rflp technique at cryptosporidium oocyst wall protein ( cowp ). fecal samples from 158 calves aged 1-20 weeks were collected from 10 d...

background: addition of amino acids to the culture medium is beneficial for embryonic development in many species. objective: the objective of this study was to investigate the effects of amino acids on the in vitro maturation and embryonic development of the bovine oocyte. materials and methods: bovine ovaries were collected from a local abattoir and brought into laboratory. cumulus-oocyte com...

background: progress achieved in culture media formulations have resulted led to an improvement in maintaining the mammalian embryo in culture throughout the preimplantation and pre-attachment period. objective: the objective of this study was to evaluate the effect of various concentrations of myo-inositol during in vitro fertilization of bovine oocytes on subsequent embryo development. materi...

interferon gamma (ifn-γ) is one of the key cytokines in defining t helper 1 lymphocyte immuneresponses. in this study, the bovine ifn-γ gene was cloned from spleen tissue rna using the reversetranscription-polymerase chain reaction (rt-pcr). ifn-γ cdna was sub-cloned and expressed inmammalian expression plasmid (pcdna3.1(+)) under the control of the human cytomegalovirus (cmv)promoter. the pred...

Aim The study was designed to measure the seroprevalence of viral and bacterial diseases: Infectious bovine rhinotracheitis, bovine viral diarrhea, bovine leukemia, bovine parainfluenza, bovine respiratory syncytial disease, brucellosis, and paratuberculosis among bovine of Himachal Pradesh during the year 2013-2015. Materials and Methods The serum samples were collected from seven districts ...

background : bovine ephemeral fever (befv) is an arthropod-borne disease of cattle and water buffaloes. befv occurs seasonally in tropical, subtropical and temperate regions of africa, asia and australia. it has been known for the past decades in iran based on clinical signs but lack of an accurate diagnosis has made the real feature of disease obscured. this is the first scientific report on i...

conclusions the prepared antibody can be used for the development of an elisa kit to assay afm1 in milk and other biological fluids. background aflatoxins are the most extensively studied group of mycotoxins produced by molds, especially the aspergillus group, which are highly toxic to animals and humans. objectives since immunoassay is a simple and rapid method for the analysis of many toxic s...

bovine leukemia provirus: evidence of presence of part of gag gene inseminal plasma of naturally infected bullsit is of critical importance to understand the modalities of blv presence in semen, especially with regard to artificial insemination (ai). presence of bovine leukemia provirus was demonstrated in fresh and frozen semen samples by researchers. in this study paired blood and semen sampl...