1 During the last years, the use of type III secretion system-based bacterial vectors for 2 immunotherapy purpose has been assessed in various applications. We showed that a type 3 III secretion-based P. aeruginosa vector delivering the OVA antigen induced an efficient 4 specific CD8+ T lymphocyte immune response against OVA-expressing cells. Because of 5 the intrinsic toxicity of the vector, f...

direct regenerations by using mature cotyledonary node as a explants has been shown to be time-saving and convenient strategy for micropropagation of soybean. so we have evaluated regeneration protocol through single shoot using cotyledonary node as a rapid and efficient protocol for two soybean cultivars and one mutant line. cotyledonary nodes explants obtained from 7-days-old in vitro seedlin...

Nonfused (i.e., nonhybrid) filamentous hemagglutinin (FHA) of Bordetella pertussis was efficiently expressed in Escherichia coli K-12 and Salmonella typhimurium aroA at levels higher than those found in wild-type B. pertussis when the upstream signals of the gene were replaced and the translation initiation region was engineered to optimize translational efficiency. Inclusion of part of the C-t...