The conventional identification (assimilation profiles in the API 20C [bioMérieux Vitek, Inc., Hazelwood, Mo.] identification system, colony color on CHROMagar Candida [CHROMagar, Paris, France], and morphology on cornmealTween 80 agar) of 32 atypical isolates of Candida albicans recovered during a study of oropharyngeal candidiasis in human immunodeficiency virus-infected individuals could not...

Background: The emergence and re-emergence of antibiotic-resistant foodborne bacteria call for concerted efforts, especially in developing countries. Spices have been employed traditionally to improve the aroma and flavour of foods; however, they could  serve as reservoirs for the spread of potential pathogenic organisms. This study aims to investigate the public health implications of antibiot...

The API ZYM reactions of type species of Gram-negative anaerobes representative of those encountered in human infections and of 56 clinical isolates of such organisms, identified by conventional techniques, were investigated. The API ZYM test clearly distinguished between the different genera and species examined and appears to provide a simple, reliable method for the identification of this gr...

One hundred strains of Enterobacteriaceae were examined in parallel with the API Rapid 20E and Micro-ID commercial four hour identification systems. With the API Rapid 20E system 78% of the strains were correctly identified, 15% were not identified, and 7% were misidentified. The respective figures with the Micro-ID system were 74%, 11%, and 15%.

The biotypes of Haemophilus influenzae and Haemophilus parainfluenzae isolates were determined with three commercially available biochemical test kits: the IDS RapID NH system, the Neisseria-Haemophilus identification test (NHI card), and the API NH strip. The API NH strip performed best, correctly classifying the biotypes of 371 of 380 (97.6%) different challenge strains.

Polymorphs of an Active Pharmaceutical Ingredient (API) can have different chemical and physical properties and, thus, a direct impact on the processability of the drug substance and the quality/performance of the drug product, such as stability, dissolution, and bioavailability. Therefore, identification of the crystal phase of an API is of outmost importance. In this work methodologies were d...

A total of 137 strains of Gardnerella vaginalis were examined by the API 20 Strep system. The system was shown to be reliable when the tests were compared with standard identification methods, and very little confusion occurred with streptococcal profiles; consequently, G. vaginalis has been included in the API 20 Strep data base.

Coagulase-negative staphylococci (CNS) species identification is still difficult for most clinical laboratories. The scheme proposed by Kloos and Schleifer and modified by Bannerman is the reference method used for the identification of staphylococcal species and subspecies; however, this method is relatively laborious for routine use since it requires the utilization of a large number of bioch...

Enteric-Tek (Flow Laboratories, Inc., Roslyn, N.Y.) is a new system designed for the identification of Enterobacteriaceae and consists of a round, multicompartmented plastic plate with 11 peripheral wells and 1 center well incorporating 14 biochemical reactions. Only one reagent (Kovacs reagent) is required to complete the biochemical test results for generation of a five-digit number for compu...