نتایج جستجو برای: antibody sandwich enzyme
تعداد نتایج: 403085 فیلتر نتایج به سال:
cucumber mosaic virus (cmv) is one of widely-spread viruses of plants with the broadest host range encompassing over 1200 species. one major limiting factor for detection of the virus is unavailability of the virus-specific antibodies especially in developing countries. recombinant dna technology facilitates antibody preparation without requiring special equipment. in this study, coat protein (...
BACKGROUND Sandwich enzyme-linked immunosorbent assay (ELISA) is one of the most frequently employed assays for clinical diagnosis, since this enables the investigator to identify specific protein biomarkers. However, the conventional assay using a 96-well microtitration plate is time- and sample-consuming, and therefore is not suitable for rapid diagnosis. To overcome these drawbacks, we perfo...
A monoclonal antibody (MAb) immunoglobulin G2a (2125) was produced against a 60-kDa Legionella heat shock protein (HSP), recognizing a unique epitope common to all species of the genus Legionella. The antibody reacted in the immunoblot with 59 Legionella species and serogroups that were tested and showed no cross-reactivity with other bacteria, including Acinetobacter spp., Bordetella spp., Pse...
In this study, it was aimed to reveal some viruses that are a problem in tomato production areas the Ereğli district of Konya, where is common. For reason, 2019 season, leaf samples were taken from 160 plants showing symptoms similar virus disease such as mosaic, deformations, yellowing, blight on leaves and stunted plants. Possible infections Cucumber mosaic (CMV), Tomato spotted-wilt (TSWV) (...
We describe an amplified enzyme-linked immunoassay of human proinsulin in serum that detects intact proinsulin and both the 32/33 and 65/66 split forms. The method uses the IgG fraction of a polyclonal antibody raised in a guinea pig against intact proinsulin, which we used to coat plastic microtiter plates. A sandwich was formed with proinsulin by using a monoclonal antibody against C-peptide ...
Norovirus (NoV) capsid proteins were expressed as virus-like particles (VLPs) by using recombinant baculovirus in insect cells, which had 5 genotypes in genogroup I and 11 genotypes in genogroup II, and the VLPs were used as immunogens. Polyclonal antibody against the VLP of GII/3 genotype showed broad-range cross-reactivity, reacting not only with intra-genogroup strains, but also inter-genogr...
The development of sensitive and selective assays for protein biomarkers other biological analytes is important advancing the fields clinical diagnostics bioanalytical chemistry. potential advantages using aptamers in electrochemical sandwich are being increasingly recognized. These may include an aptamer as both capture detection agent or a combination with different partner such antibody, lec...
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