Amoebic keratitis, a sight-threatening corneal infection, mainly occurs in contact lens wearers who wash their eyes with tap water. The present research was conducted to identify the occurrence of potentially pathogenic free-living amoebae (FLA) in tap water sources on Kish Island, a tourist region in Iran. Amoebae were detected using a culture-enriched method and by polymerase chain reaction (...

The free-living amoebae Naegleria species (spp.) have been recognized as etiologic agents of amoebic encephalitis, keratitis, otitis, lung lesions and other skin infections mainly in immuno-compromised individuals. In this study, morphological and biochemical characterization of Naegleia strains isolated from the Egyptian aquatic environment were surveyed. Some Naegleria species were cultivated...

The amphizoic protozoa Acanthamoeba species are able to be free-living amoebae in environment or as parasites in humans and animals. These species comprise nearly 30 species and they have an increased role as human pathogens causing encephalitis in the nervous system or keratitis in the eyes. In diagnosis of a clinical sample of eye contact lens, light microscopy showed a heavy growth of amoebo...

Acanthamoeba, a free-living amoeba, is widely distributed in the environment, water sources, soil, dust, and air. It can cause keratitis in contact lens wearers with poor hygiene and also fatal granulomatous amebic encephalitis (GAE) in immunocompromised hosts. The aim of this study was to gain some insights into the distribution and genotypes of the potentially pathogenic species of Acanthamoe...

Acanthamoeba keratitis (AK) is a potentially blinding infection caused by protozoa found worldwide. The topical application of biguanides and diamidines the most common anti-amoebic treatment for AK. In this study, we hypothesized that geographical location socioeconomic status influence management To test hypothesis, analyzed case reports series eye infections from different geographic regions...

The effects of clinically available drugs targeting muscarinic cholinergic, adrenergic, dopaminergic, and serotonergic receptors; intracellular calcium levels and/or the function of calcium-dependent biochemical pathways; ion channels; and cellular pumps were tested against a keratitis isolate of Acanthamoeba castellanii belonging to the T4 genotype. In vitro growth inhibition (amoebistatic) as...

A micro enzyme-linked immunosorbent assay was used to detect the presence of amoebic antigen in polyethylene-glycol-precipitated circulating immune complexes (CIC). A cutoff dilution of greater than 1:160 for the precipitates was taken to be of clinical significance. Among the patients with amoebic liver abscess, 93% (14 of 15 confirmed cases) had indications of amoebic antigen in CIC. In 57% o...

Amoebic liver abscess is one of the forms invasive amoebiasis. With untimely treatment, this disease can be complicated by peritonitis, dissemination pathogen with development sepsis. This article presents a clinical case amoebic in patient V., 40 years old results biopsy examination surgical specimens

An IgG ELISA was evaluated as a serological test for the diagnosis of invasive amoebic disease in an endemic area. Sixteen patients with proven amoebic dysentery, 22 with suspected amoebic dysentery and 21 with amoebic liver abscesses were studied. Control subjects were 26 asymptomatic amoebic cyst passers, 13 patients with giardiasis, 26with enteric fever and 31 individuals with no evidence of...

Amoebic appendicitis is very rare, occurring in about 0.5% to 2% of acute appendicitis, and usually not reported in children. We report a case of confirmed acute amoebic appendicitis complicating amoebic colitis in a 7-year-old Pakistani boy living in southern Jeddah, Saudi Arabia, and briefly review the literature on parasitic appendicitis.