Fructose-1,6-bisphosphate aldolase from Drosophila melanogaster has been crystallized from polyethylene glycol 6000 by vapor diffusion technique against buffered polyethylene glycol solutions at 2-4 degrees C. The insect enzyme crystallizes in the orthorhombic system, heretofore unknown for aldolases. The crystals have the space group P212121 (a = 86.3 A, b = 115.7 A, and c = 151.4 A) and conta...

Experiments with D-glucose-6-P stereospecifically tritiated at C-6 showed that the myo-inositol-1 P synthase reaction catalyzed by both the enzyme from beef testis and from pollen of Lilium longiflorum proceeds with stereospecific loss of the pro-6R and incorporation of the pro-6S hydrogen into the product. The ring closure thus occurs in a retention mode at C-6 of the substrate, a finding at v...

The methodology for the preparation of cross-linked enzyme aggregates (CLEAs) and their application as highly effective, recyclable biocatalysts in a wide variety of transformations is reviewed. The method constitutes a combination of enzyme purification and immobilization into a single operation and allows for the use of relatively crude enzyme preparations. The resulting CLEAs have excellent ...