Kinetic analyses of lactate-dehydrogenase (LD)-coupled alanine transaminase (ALT) reaction processes were investigated for measuring ALT by an integration strategy. For measuring ALT by a kinetic analysis of an LD-coupled ALT reaction curve, candidate reaction curves were calculated via iterative numerical integration of the differential velocity equations to execute a weighted nonlinear-least-...

OBJECTIVE This study examined the risk factors associated with failure of enhanced recovery protocol after major hepatobiliary and pancreatic (HBP) surgery. SETTING AND PARTICIPANTS A retrospective cohort of 194 adult patients undergoing major HBP surgery at a university hospital in Hong Kong was followed up for 30 days. The patients were from a larger cohort study of 736 consecutive adults w...

BACKGROUND The aim of this study was to establish the reference intervals (RIs) of total bilirubin (TBIL), alanine aminotransferase (ALT), aspartate transaminase (AST), and creatinine (CREA) for apparently healthy elderly (Han ethnicity) in Shuyang, China. MATERIAL AND METHODS A total of 54 912 blood specimens from elderly residents age 65-104 years were collected by standard procedures in Sh...

The stability of transaminase enzymes in the filter paper matrix, at different temperatures for different lengths of time is of considerable importance. A method for extraction and estimation of theses enzymes from serum spotted on filter paper (whatman no.3) has been developed. The enzymes were efficiently extracted and measured from dried serum spots. For estimation, a commercially available ...

BACKGROUND To develop a screening algorithm to detect hepatic steatosis in overweight and obese adolescents. METHODS We performed a cross sectional study of 129 overweight adolescents 13-18 yrs. The primary outcome, hepatic steatosis was defined as an intracellular triglyceride content > 5.5 mg/g and quantified using (1)H-magenetic resonance spectroscopy. Primary predictor variables included,...

OBJECTIVE To investigate the risk of hepatotoxicity after initiation of protease inhibitor-containing highly active antiretroviral therapy (HAART) for HIV-1 infected patients with chronic hepatitis B virus (HBV) or hepatitis C virus (HCV) co-infection. DESIGN Retrospective study with 394 HIV-1-infected patients initiating HAART at a single university clinic. METHODS Liver enzyme elevation (...

To evaluate pretreatment of six polyherbal liquid formulations (PLFs) commercially available in India, on CCl4-induced liver injury, Swiss albino mice were treated for 7 days with distilled water or PLFs (2.6 and 5.2 ml/kg body weight/day, po) followed by single sc injection of 50% (v/v) CCl4 in arachis oil at a dose of 1 ml/kg. The serum biochemical parameters such as alanine transaminases, as...

OBJECTIVE To evaluate the associations between adverse perinatal outcomes and serum transaminase levels at the time of diagnosis in patients with intrahepatic cholestasis of pregnancy. METHODS We performed a retrospective analysis of patients hospitalized for evaluation of intrahepatic cholestasis of pregnancy from January 2013 to June 2014 in a tertiary center. Seventy-one patients were divi...

OBJECTIVE We sought to examine the association between plasma concentrations of liver enzymes gamma-glutamyltransferase (GGT) and alanine transaminase (ALT) and incident diabetes, prospectively. RESEARCH DESIGN AND METHODS We conducted a case-cohort analysis of data from participants mainly aged 35-65 years in the European Prospective Investigation into Cancer and Nutrition-Potsdam Study. The...

Abnormalities in liver function tests, including transient and self-limiting hypertransaminasemia, cholestatic disease and hepatitis, can develop during treatment with anti-tumour-necrosis-factor (TNF) therapy. The optimal management of liver injury related to anti-TNF therapy is still a matter of debate. Although some authors recommend discontinuing treatment in case of both a rise of alanine ...