the Centre. Pseudo inflammatory, bilateral, or multiple cancers were excluded from the study. The stage of the disease was classified according to the TNM (tumors-nodes-metastasis) system (19). Treatment consisted of either modified partial mastectomy (1 6) with ablation of the axillary nodes or partial mastectomy with low axillary dissection (Levels 1 and 2 of Berg), followed by postsurgical r...

The study was designed with an aim to evaluate the protective effects of reduced glutathione on 5fluorouracil (5-FU)-induced Cholesterol profile in vitro. Goat blood was used as lipid source for the model. In the cholesterol profile total cholesterol (TC) and high density lipoprotein (HDL) cholesterol content of goat blood was determined. 5-FU and reduced glutathione were added at a concentrati...

PURPOSE The International Organization for Standardization-5fluorouracil (FU) 10 trial found that bolus 5-FU and l-leucovorin was not inferior to S-1 in the treatment of gastric cancer (GC). Continuous 5-FU and the rapid injection of 5-FU have different anti-cancer effects. Thus, bolus 5-FU and l-leucovorin treatment might be useful for oral FU-resistant GC. MATERIALS AND METHODS We retrospec...

The variable number of tandem repeat (VNTR) of thymidylate synthase (TS) gene, mainly 2 repeat (2R) and 3 repeat (3R), is one of the genetic variations that can potentially predict the effectiveness of 5-fluorouracilbased chemotherapy. In this study we identified an additional single nucleotide polymorphism (SNP) in the VNTR of TS, followed by functional and clinical analysis of the SNP. Two-hu...

Proteomic analysis of biological samples is increasing in its importance for various research applications. Part and parcel of this increase in interest is an influx of commercial formats available for utilizing this technology. Because cost and application are generally limiting factors when choosing among the various instrumentation available, one challenge for a core facility is then to choo...

Polyamines have been shown to play a role in the growth and survival of several solid tumors, including colorectal cancer. We identified the polyamine catabolic enzyme spermidine/spermine N-acetyltransferase (SSAT) as being one of the most highly inducible genes in two DNA microarray screens to identify novel determinants of response to chemotherapeutic agents in colorectal cancer. SSAT was sho...

VIPomas are rare neuroendocrine tumors that secrete vasoactive intestinal peptide. They are detected in 1 in ten million people per year. The majority of these tumors arises within the pancreas, but may also arise as other VIP-secreting tumors as in bronchogenic carcinoma, colon carcinoma, ganglioneuroblastoma, pheochromocytoma, hepatoma and adrenal tumors. They are characterized by profound di...

The management of rectal cancer requires a multidisciplinary approach with individual treatment based on a careful assessment of tumour location, stage and resectability. Early identification of the patients that are candidates for combined modality treatment is crucial. After showing less acute and long-term toxicity along with an improved local control in a randomized study, pre-operative com...

We have demonstrated previously that interferon (IFN)-gamma sensitizes human colon carcinoma cell lines to the cytotoxic effects of 5-fluorouracil combined with leucovorin and to the thymidylate synthase inhibitor, ZD9331, dependent on thymineless stress-induced DNA damage, independent of p53. Here we demonstrate that the cyclin-dependent kinase (CDK) inhibitor p21(Cip1) regulates thymineless s...

To the Editor: In their important article, Ezzeldin et al. (1) report a positive association between dihydropyrimidine dehydrogenase (DPD) deficiency and the DPD gene (DPYD) promoter hypermethylation in peripheral blood mononuclear cells of studied individuals. The authors focused on methylation in the individuals with the DPD deficiency, but did not elaborate on the impact of hypermethylation ...