نتایج جستجو برای: 2dg electrophoresis

تعداد نتایج: 50889  

Journal: :PloS one 2016
Meliana Riwanto Sarika Kapoor Daniel Rodriguez Ilka Edenhofer Stephan Segerer Rudolf P Wüthrich

Dysregulated signaling cascades alter energy metabolism and promote cell proliferation and cyst expansion in polycystic kidney disease (PKD). Here we tested whether metabolic reprogramming towards aerobic glycolysis ("Warburg effect") plays a pathogenic role in male heterozygous Han:SPRD rats (Cy/+), a chronic progressive model of PKD. Using microarray analysis and qPCR, we found an upregulatio...

Journal: :American journal of physiology. Regulatory, integrative and comparative physiology 2005
A J Thorpe J A Teske C M Kotz

Orexin neurons are stimulated by conditions that are glucoprivic, suggesting that orexin signaling may be increased during nutritional duress. We have previously shown that injection of orexin A (OxA) into the rostral lateral hypothalamic area (rLHa) robustly and dose-dependently increases feeding behavior. Thus we hypothesized that exogenous administration of orexin A would induce a greater fe...

Journal: :The Journal of neuroscience : the official journal of the Society for Neuroscience 2008
Anna Devor Elizabeth M C Hillman Peifang Tian Christian Waeber Ivan C Teng Lana Ruvinskaya Mark H Shalinsky Haihao Zhu Robert H Haslinger Suresh N Narayanan Istvan Ulbert Andrew K Dunn Eng H Lo Bruce R Rosen Anders M Dale David Kleinfeld David A Boas

The present study addresses the relationship between blood flow and glucose consumption in rat primary somatosensory cortex (SI) in vivo. We examined bilateral neuronal and hemodynamic changes and 2-deoxyglucose (2DG) uptake, as measured by autoradiography, in response to unilateral forepaw stimulation. In contrast to the contralateral forepaw area, where neuronal activity, blood oxygenation/fl...

2011
Ai-Jun Li Qing Wang Sue Ritter

OBJECTIVE To examine the role of AMP-activated protein kinase (AMPK) in the control of glucoprivic feeding by hindbrain catecholamine neurons. RESEARCH DESIGN AND METHODS Micropunched hindbrain samples were collected from control and 2-deoxy-d-glucose (2DG)-injected rats for Western blot analysis of phosphorylated (activated) AMPK (pAMPK). Samples also were collected from 2DG-injected rats pr...

2014
Grigorios Spirakis Konstantinos Nikolopoulos

Authors: Grigorios Spirakis, School of Business and Economics, University of Peloponnese, Tripolis 22100, Greece; Antonios Sarantidis, Visiting Researcher, Forecasting Laboratory, Bangor Business School, Hen Coleg, College Road, Bangor, LL57 2DG, United Kingdom; Konstantinos Nikolopoulos, Bangor University, Business School, Hen Coleg, College Road, Bangor, LL57 2DG, United Kingdom, Kostas@forta...

2017
Sarah Niccoli Douglas R Boreham Christopher P Phenix Simon J Lees

A glucose analog called 2-deoxy-D-glucose (2DG) has been successfully used to sensitize cancer cells to ROS-inducing cancer treatments such as ionizing radiation, through the inhibition of glycolysis. However, the use of 2DG can be limited by several factors such as availability, non-specific cytotoxicity, and chemoresistance under hypoxic conditions. The purpose of this study was to investigat...

Journal: :Journal of immunology 2012
Lars Andresen Sarah Line Skovbakke Gry Persson Michael Hagemann-Jensen Karen Aagaard Hansen Helle Jensen Søren Skov

NKG2D ligand surface expression is important for immune recognition of stressed and neotransformed cells. In this study, we show that surface expression of MICA/B and other NKG2D ligands is dependent on N-linked glycosylation. The inhibitor of glycolysis and N-linked glycosylation, 2-deoxy-D-glucose (2DG), potently inhibited surface expression of MICA/B after histone deacetylase inhibitor treat...

Journal: :Brain : a journal of neurology 2006
R Jarrett Rushmore Antoni Valero-Cabre Stephen G Lomber Claus C Hilgetag Bertram R Payne

Visuospatial neglect is a common neurological syndrome caused by unilateral brain damage to the posterior and inferior parietal cerebral cortex, and is characterized by an inability to respond or orient to stimuli presented in the contralesional hemifield. Neglect has been elicited in experimental models of the rat, cat and monkey, and is thought to result in part from a pathological state of i...

2015
Iben Lundgaard Baoman Li Lulu Xie Hongyi Kang Simon Sanggaard John Douglas R Haswell Wei Sun Siri Goldman Solomiya Blekot Michael Nielsen Takahiro Takano Rashid Deane Maiken Nedergaard

Metabolically, the brain is a highly active organ that relies almost exclusively on glucose as its energy source. According to the astrocyte-to-neuron lactate shuttle hypothesis, glucose is taken up by astrocytes and converted to lactate, which is then oxidized by neurons. Here we show, using two-photon imaging of a near-infrared 2-deoxyglucose analogue (2DG-IR), that glucose is taken up prefer...

2015
Siwen Li Aqin Wang Fan Gu Zhaohui Wang Caiping Tian Zhiyu Qian Liping Tang Yueqing Gu

Harmine is a beta-carboline alkaloid found in medicinal plant PeganumHarmala, which has served as a folk anticancer medicine. However, clinical applications of harmine were limited by its low pharmacological effects and noticeable neurotoxicity. In this study, we modified harmine to increase the therapeutic efficacy and to decrease the systemic toxicity. Specifically, two tumor targeting harmin...

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