Abstract During the 2019 Eastern equine encephalitis virus (EEEV) outbreak in Michigan, two 2-month old Mexican wolf pups experienced neurologic signs, lymphohistiocytic neutrophilic meningoencephalitis with neuronal necrosis and neuronophagia, acute death. We identified EEEV by reverse transcription real-time PCR situ hybridization. Vector mosquitoes were trapped at zoo.

BACKGROUND In most measurements of gene expression, mRNA is first reverse-transcribed into cDNA. We studied the reverse transcription reaction and its consequences for quantitative measurements of gene expression. METHODS We used SYBR green I-based quantitative real-time PCR (QPCR) to measure the properties of reverse transcription reaction for the beta-tubulin, glyceraldehyde-3-phosphate deh...

During retroviral DNA synthesis reverse transcriptase frequently performs nonrequired template switches that can lead to genetic rearrangements or recombination. It has been postulated that template switching occurs after pauses in the action of reverse transcriptase. Hence factors which affect pausing, such as polymerization rate, may affect the frequency of template switching. To address the ...

Manipulation of stress related transcription factors to improve plant stress tolerance is a major goal of current biotechnology researches. MYC2 gene encodes a key stress-related transcription factor involved in Jasmonate (JA) and abscisic acid (ABA) signaling pathways in Arabidopsis. Brassica napus, as a globally important oilseed crop, is a close relative of Arabidopsis.  In the present study...

Abstract Apple stem grooving virus, the causal agent of citrus tatterleaf disease, was detected for first time in Lao People’s Democratic Republic. Samples were collected from trees across southern provinces testing Australia. RNA extracted and tested using conventional real-time reverse transcription polymerase chain reactions with virus 1 59 samples tested. Viral identity confirmed by Sanger ...

Aim: to investigate the effect of teichoic acid Staphylococcus aureus for expression pro-inflammatory cytokines and TLR4 in a human small cell lung carcinoma line NCI-H69, cisplatin resistant subline NCI-H69/CPR. Methods. Incubation cells with (1 ng/m) conducted 2 days. Expression level TLR4, TGF-β, INF-γ, TNF-α was evaluated by real time PCR on 7500 Real-Time System, using specific primers flu...