Torularhodin is a fungus-derived carotenoid, and the lack of downstream processing torularhodin still challenge for its large-scale production. To support industrial production torularhodin, this work initially evaluated efficiency carotenoid release from Rhodotorula mucilaginosa using thermal acid treatment, saponification ultrasound-assisted enzymatic lysis. Based on polarity, was then purifi...

Introduction. Tumor lysis syndrome is an emergency condition requiring prompt recognition and treatment. It?s a consequence of spontaneous or therapy-induced cellular death leading to the release intracellular ions metabolic products purine bases into bloodstream. Pathophysiology. The characteristic derangement comprises hyperkalemia, hyperphosphatemia, hyperuricemia, hypocalcemia. These change...

Single-cell genomics (SCG) is a recently developed tool to study the genomes of unculturable bacterial species. SCG relies on multiple-strand displacement amplification (MDA), PCR, and next-generation sequencing (NGS); however, obtaining sufficient amounts of high-quality DNA from samples is a major challenge when performing this technique. Here we present an improved bacterial cell lysing proc...

Groman, Neal B. (University of Washington, Seattle). Factors in lysis and lysis inhibition by lambda bacteriophage. J. Bacteriol. 90:1563-1568. 1965.-Induced Escherichia coli strain K-12(lambda112) exhibited lysis inhibition at 37 C but lysed at 44 C when incubated in LB medium lacking NaCl [LB - (NaCl)]. In LB medium containing NaCl, the temperatures for lysis and lysis inhibition were reverse...

T4 bacteriophage (phage)-infected cells show a marked increase in latent-period length, called lysis inhibition, upon adsorption of additional T4 phages (secondary adsorption). Lysis inhibition is a complex phenotype requiring the activity of at least six T4 genes. Two basic mysteries surround our understanding of the expression of lysis inhibition: (i) the mechanism of initiation (i.e., how se...

a male patient with acute renal failure (arf) due to large b-cell non-hodgkin lymphoma infiltration of kidney is presented. the diagnosis was suspected because of coincidence of arf and tumor lysis syndrome non-responsive to conservative renal therapies. a renal biopsy confirmed diagnosis and appropriate chemotherapy led to complete improvement of renal function.

determination of streptokinase activity is usually accomplished through two assay methods: a) clot lysis, b) chromogenic substrate assay. in this study the biological activity of two streptokinase products, namely streptase®, which is a native product and heberkinasa®, which is a recombinant product, was determined against the third international reference standard using the two forementioned a...