The present investigation was carried out to assess the effect of Sephadex (G-15) filtration on the post thaw bull semen quality and conception rate. Post thaw unfiltered (control) and Sephadex filtered semen from four healthy bulls (three cross bred and one pure bred Holstein Friesian) were subjected to microscopic examination viz. sperm concentration, individual motility, live sperm count and...

One ejaculate from each Of 13 bulls was used to determine the influence of glycerol level on post-thaw susceptibili ty of spermatozoa in French straws to cold shock. Treatments were arranged factorially and included yolk-Tris and yolk-citrate diluents; 7, 9 and 11% glycerol ;and exposure to 1, 20 or 37 C water baths for 3 rain immediately post-thaw. Percentage of motile spermatozoa and percenta...

Cryopreservation protocols are essential for stem cells storage in order to apply them in the clinic. Here we describe a new standardized cryopreservation protocol for GABAergic neural precursors derived from the medial glanglionic eminence (MGE), a promising source of GABAergic neuronal progenitors for cell therapy against interneuron-related pathologies. We used 10% Me2SO as cryoprotectant an...

Soft tissue allografts, such as the bone-patellar tendon-bone (BPTB) graft, have been frequently used for anterior cruciate ligament (ACL) reconstruction. As allografts are subjected to freezing and thawing for multiple cycles, the objective of this study was to measure the changes of the biomechanical properties of the human BPTB allograft after 4 and 8 freeze-thaw cycles in comparison to a si...

OBJECTIVE The aim of the present study was to evaluate three thawing rates on the post thaw motility, viability and chromatin structure of buffalo semen frozen in 0.5-ml straws. MATERIALS AND METHODS In this experimental study semen was collected with artificial vagina (42℃) from four buffalo bulls.Split pooled ejaculates (n=4) were extended at 37℃ with a Bioxcell® extender. Semen was cooled ...

The use of superabsorbent polymers (SAPs) to produce internally cured concrete has been shown to be effective in reducing the potential for restrained shrinkage cracking of high performance concrete mixtures. However, not much is known regarding the freeze–thaw durability of concrete mixtures that incorporate SAPs for internal curing (IC). When SAP particles desorb (or partially desorb) the “wa...

Biochar as an organic soil conditioner has colossal application potential. Many recent studies revealed the influence of biochar on during crop growth period. However, few considered effect seasonal freeze-thaw biochar’s improvement effect. Therefore, we conducted a field experiment to observe impact and cotton (Gossypium hirsutum L.). We used four rates (0.33%, 0.66%, 1.00%, 1.33% mass fractio...

Nitrous oxide emissions during freeze/thaw periods contribute significantly to annual soil N2O emissions budgets in middle- and high-latitude areas; however, the freeze/thaw-related N2O emissions from waterlogged soils have hardly been studied in the Hulunber Grassland, Inner Mongolia. For this study, the effects of changes in land use/cover types on N2O emissions during freeze-thaw cycles were...

OBJECTIVES Progastrin-releasing peptide (proGRP) is a promising biomarker for small cell lung cancer. However, not much is known about how sample processing and storage conditions affect the stability of proGRP. Here, we examined the effects of repeated freeze-thaw cycles on the stability of proGRP in plasma and serum. METHODS Concentrations of proGRP were measured in plasma and serum samples...