The accelerating pace of genomics analysis has necessitated the abbreviation of DNA sample preparation protocols. We have developed a size-exclusion-based system for the rapid isolation ofplasmid DNA in a 96-well microplate format. This high-speed protocol employs a modified alkaline lysis methodfor the preparation of the bacterial lysate, followed by three short vacuum filtration steps. Unlike...

A series of novel chiral triazolium salts has been synthesized from readily available (1R,2R)-DPEN and found to be efficient for the enantioselective intramolecular Stetter reaction. With 10 mol% of the catalyst, the intramolecular Stetter reaction was realized in excellent yields with up to 97% ee.

Anti-leukemia screening of previously prepared isothiouronium and quaternary salts was performed, and some salts exhibited promising activity as anticancer agents. Quantum chemical calculations were utilized to explore the electronic structure and stability of these compounds. Computational studies have been carried out at the PM3 semiempirical molecular orbitals level, to establish the HOMO-LU...

Force-extension curves (FECs), which quantify the response of a variety of biomolecules subject to mechanical force (f), are often quantitatively fit using worm-like chain (WLC) or freely jointed chain (FJC) models. These models predict that the chain extension, x, normalized by the contour length increases linearly at small f and at high forces scale as x ~ (1 - f(-α)), where α = 0.5 for WLC a...

The radical-mediated addition of triphenylphosphonium tetrafluoroborate to olefins (hydrophosphonation) is reported. Both standard radical initiators and photochemical conditions are effective, up to the gram scale. The phosphonium salts are shown to serve as Z-selective Wittig olefination reagents, even without purification.

The detection and identification of specific molecules may often be accomplished most conveniently by autographic methods. These procedures, in which nutritionally deficient bacterial cells suspended in solid minimal media show zones of growth in the presence of the required nutrilite, possess several advantages over liquid assay methods. These Include simplicity of technique and the reduction ...

2-Silaimidazolium ions and 2-silaimidazolidinium ions were synthesized by Lewis acid-base adduct formation between N-heterocyclic silylenes and silyl arenium ions. They were isolated in high yields as their [B(C(6)F(5))(4)](-) salts. These salts are stable at room temperature and were characterized by NMR spectroscopy supported by the results of density functional computations of molecular stru...

The problem of DNA- DNA interaction mediated by divalent counterions is studied using a generalized grand-canonical Monte-Carlo simulation for a system of two salts. The effect of the divalent counterion size on the condensation behavior of the DNA bundle is investigated. Experimentally, it is known that multivalent counterions have strong effect on the DNA condensation phenomenon. While tri- a...

We propose a quantitative estimate of the charge gap that opens in the onedimensional dimerized Hubbard model at quarter-filling due to dimerization, which makes the system effectively half–filled, and to repulsion, which induces umklapp scattering processes. Our estimate is expected to be valid for any value of the repulsion and of the parameter describing the dimerization. It is based on anal...

The synthesis, single crystal X-ray structure and activity in olefin metathesis of novel anionic tungsten imido alkylidene complexes [1,3-bis-(2,4,6-trimethylphenyl)imidazolinium](+) [W(N-2,6-iPr2C6H3)(CHCMe2Ph)(2,5-Me2Pyr)2Cl](-), [1,3-bis-(2,4,6-trimethylphenyl)imidazolinium](+) [W(N-2,6-iPr2C6H3)(CHCMe2Ph)(2,5Me2Pyr)2(OC6F5)](-), and [1,3-bis-(2,6-diisopropylphenyl)imidazolinium](+) [W(N-2,6...