Corn (Zea mays L.) fiber, which is the seed coat and residual endosperm left after grain processing, is a low-value residue that contains carbohydrates and aromatic compounds that could provide value-added coproducts. Treatment of corn fiber with NaOH and assessment by gas chromatography indicated a prevalence of ferulic acid, with about 90% ester-linked in the cell walls. p-coumaric acid was m...

The effect of tartrazine on prostaglandin production was evaluated in several in vitro systems in order to elucidate the interrelationship between aspirin-sensitive asthma and tartrazine. Unlike the nonsteroidal anti-inflammatory drugs, tartrazine did not inhibit cyclooxygenase activity in sheep seminal vesicles, guinea pig lung microsomes, and human platelets. Tartrazine had no effect on the a...

During surveys of amines in the urine of children, Perry et al. (1) and Perry and Schroeder (2) found among the unidentified free bases present a substance which produced an orange-red color when chromatograms were sprayed with diazotized sulfanilic acid. This substance was designated “Compound 9.” Subsequently Compound 9 has been detected in all amine concentrates of unhydrolyzed urines of a l...

HE PURPOSES OF THE PRESENT PAPER are to describe some new sensitive tests suitable for the detection of bile in biologic fluids which overcome the defects demonstrated in procedures described in most standard texts, and also to confirm the experiments of C. E. May et at. (1). The sensitivity of the proposed oxidation tests to detect bilirubin, viz. ferricyanide, molybdate, N-bromosuccinimide (2...

The preparation of undenatured apocytochrome bs and the apoprotein of an iodinated and acetylated derivative of cytochrome bs made possible the examination of the characteristics of hemei binding in this heme protein (2). A specific imidazole group on the apocytochrome was implicated in this reaction by stoichiometric titration of this single group on the iodinated and acetylated apoprotein wit...

Isolation on a preparative scale of thyrotropin-releasing rormone from porcine hypothalami is described. The yields ‘ram two batches of 100,000 and 165,000 hypothalami were !.8 mg and 4.4 mg, respectively. The material with thyro:ropin-releasing hormone activity, calculated to be purified Ibout 570,000 times, was active in mice at doses of 1 ng in jiuo, and stimulated the secretion of thyroid-s...

Seventeen commonly used dyes and 16 of their metabolites or derivatives were tested in the Salmonella-mammalian microsome mutagenicity test. Mutagens active with and without added Aroclor-induced rat liver microsome preparations (S9) were 3-aminopyrene, lithol red, methylene blue (USP), methyl yellow, neutral red, and phenol red. Those mutagenic only with S9 activation were 4-aminopyrazolone, 2...