Tetracycline or doxycycline (dox)-regulated control of genetic elements allows inducible, reversible and tissue specific regulation of gene expression in mice. This approach provides a means to investigate protein function in specific cell lineages and at defined periods of development and disease. Efficient and stable regulation of cDNAs or non-coding elements (e.g. shRNAs) downstream of the t...

Impulsivity, a tendency toward immediate action without consideration of future consequences, is associated with a wide array of problematic behaviors. Response impulsivity, a type of behaviorally-assessed impulsivity characterized by behavioral disinhibition, is also associated with health risk behaviors. Response impulsivity is distinct from choice impulsivity, which is characterized by intol...

If you study a human disease, it is likely that you have tried to generate a mouse model. Sometimes, these models are excellent; others are disappointing. Or, so we think. How often does our mouse mutant not model the human disease because of limitations in how we may look at it? In any living organism, many factors work together to produce the phenotype. Here, new phenotyping paradigms for ass...

Both wild-type (WT) and nonconducting W472F mutant (NCM) Kv1.5 channels are able to conduct Na(+) in their inactivated states when K(+) is absent. Replacement of K(+) with Na(+) or NMG(+) allows rapid and complete inactivation in both WT and W472F mutant channels upon depolarization, and on return to negative potentials, transition of inactivated channels to closed-inactivated states is the fir...

It has been suggested that the collaborative cross, a large set of recombinant inbred strains derived from eight inbred mouse strains, would be a powerful resource for the dissection of complex phenotypes. Here we use simulation to investigate the power of the collaborative cross to detect and map small genetic effects. We show that for a fixed population of 1000 individuals, 500 RI lines bred ...

Using mAb that selectively recognize the various allelic forms of the VHT15 and Vk21D-E genes' products, we analyzed the influence of VH and Vk polymorphism on the probability of expression of these gene segments. Our data show that the frequency to which the VHT15 gene product becomes available in the preimmune repertoire is strongly influenced by the polymorphism of the relevant structural ge...

113 Effects of dietary non-phytate phosphorus level and phy­ tase on growth performance of Cherry Valley duck. Z. Z. Ren*1, Q. F. Zeng1, X. M. Ding1, S. P. Bai1, Y. H. Luo1, J. P. Wang1, K. Y. Zhang1, and G. Xu2, 1Institute of Animal Nutrition, Animal Nutrition and Feed Engineering, Key Laboratory of Sichuan Province, Sichuan Agricultural University, Sichuan, China, 2Sichuan Chuanheng Chemi­ ca...

The similarity between the behaviors evoked by tail pinch (TP) and electrical stimulation of the lateral hypothalamus (ESLH) has been noted by many investigators. Evidence is presented for a possible inverse relationship between the probability that ESLH would evoke eating and the readiness to eat in response to TP in six different rat populations (High and Low lines of the LC1 and LC2 Hebrew U...

Genetic variation, through its effects on gene regulatory networks, has an important role in determining biologic phenotypes and disease susceptibility. Recent studies using segregating populations have characterized variation in gene expression resulting from genetic differences and have used well-established assays to identify many cis-acting polymorphisms. Although trans effects have been sh...