Manufacture of red blood cells (RBCs) from progenitors has been proposed as a method to reduce reliance on donors. Such a process would need to be extremely efficient for economic viability given a relatively low value product and high (2 × 1012 ) cell dose. Therefore, the aim of these studies was to define the productivity of an industry standard stirred-tank bioreactor and determine engineeri...

Designing large-scale stirred bioreactors with performance closely matching the one achieved in lab-scale fermenters presents continuous challenge. In this contribution, dynamic modelling of the aerobic biocatalytic conversion process in viscous batch stirred tank reactor is developed. Its operation is illustrated by simulation of the interaction of fluid flow, mass transfer and reaction releva...

Wave-mixed bioreactors have increasingly replaced stainless steel stirred tank reactors in seed inoculum productions and mammalian cell-based process developments. Pre-sterilized, single-use plastic bags are used for cultivation, eliminating the risk of cross-contamination and cleaning procedures. However, these advantages come with high consumable costs which is the main barrier to more uptake...

The Wave Bioreactor is widely used in cell culture due to the benefits of disposable technology and ease of use. A novel cellbag was developed featuring a frit sparger to increase the system's oxygen transfer. The purpose of this work was to evaluate the sparged cellbag for yeast cultivation. Oxygen mass transfer studies were conducted in simulated culture medium and the sparged system's maximu...

An analysis of the effects of the turbulent shear stress on cell culture in a stirred tank microcarrier bioreactor system using a synergistic combination of computational fluid dynamic (CFD)-based simulations and experiments is presented. A 3D computational model of Corning’s benchscale spinner flask was built using a state-of-the-art computational fluid dynamics (CFD) software, Flow3D (www.flo...

This paper deals with the problem of modelling and on-line estimation of kinetics for a biomethanation process. This bioprocess is in fact a wastewater biodegradation process with production of methane gas, which takes place inside a Continuous Stirred Tank Bioreactor. The reaction scheme and the analysis of biochemical phenomena inside the bioreactor are used in order to obtain a nonlinear dyn...

The influence of power consumption on the volumetric mass transfer coefficient was studied in a 5 liter stirred tank bioreactor during cultivation of a recombinant Chinese Hamster Ovary (CHO) cell line, which requires low aeration and mixing intensity. Under these conditions and at high cell concentrations, oxygen mass transfer coefficient and consequently volumetric oxygen mass transfer rate w...

The effect of organic and inorganic nitrogen sources on Trichoderma reesei Rut-C30 cellulase production was investigated in submerged cultivations. Stirred tank bioreactors and shake flasks, with and without pH control, respectively, were employed. The experimental design involved the addition of individual organic nitrogen sources (soy peptone, glutamate, glycine and alanine) within a basal me...