The actin cytoskeleton plays critical roles in numerous cellular events and functions, its spatiotemporal dynamics are maintained regulated by several cofactor proteins. MISP/Caprice is a recently reported actin-bundling protein that also involved the progression of mitosis. In this study, we investigated how actin-regulatory function MISP modulated phosphorylation. A series mutation studies de...

Controlling the quantity and size of organelles through competition for a limited supply of components is quickly emerging as an important cellular regulatory mechanism. Cells assemble diverse actin filament (F-actin) networks for fundamental processes including division, motility, and polarization. F-actin polymerization is tightly regulated by activation of assembly factors such as the Arp2/3...

Abstract CD157 is an ectoenzyme that has both cyclic ADP-ribose hydrolase and ADP-ribosyl cyclase activities. In the human hematopoietic system, prevalently expressed by cells of myelomonocytic lineage, interacts with extracellular matrix components regulates leukocyte diapedesis via integrin-mediated signaling. implicated in pathophysiology several neurological disorders. Although it lacks a c...

Macrophages, cells effective in sensing, internalizing and killing Candida albicans, are intertwined with the extracellular matrix (ECM) through different signals, which include release of specific cytokines. Due to importance these interactions, employment vitro models mimicking a fungal infection scenario is essential evaluate ECM effects on macrophage response. In this work, we have analyzed...

Actin, the major protein of Naegleria gruberi, is selectively not synthesized during the differentiation of amebae to flagellates. When RNA extracted from cells at intervals during differentiation is translated in the wheat germ cell-free system, a major translation product with the electrophoretic mobility of actin is seen to disappear with time during differentiation. This translation product...

background bone marrow and adipose tissue are two main sources of mesenchymal stem cells (mscs). some of studies suggest that there are some differences in gene expression profile of mscs-derived from various tissues. to investigate gene expression profile by qrt-pcr, an appropriate reference gene with stable expression level should be chosen for normalizing data.  this study was designed to ev...