Abstract Neoantigens derived from somatic mutations in cancer cells can induce antigen‐specific T‐cell immune response for immunotherapy. However, the 3D models assessing neoepitope immunogenicity and efficacy of anti‐tumor to neoantigens are less than perfect. Here, a tumor model based on recellularized liver matrix is leveraged with HepG2 investigate T cell cytotoxic reactivity toward hepatoc...

12 rearranged human VH6 immunoglobulin heavy chain genes arising from the same rearrangement were isolated without preselection from the RNA of a fragment of human spleen. The 12 clones were isolated from a pool of 31 unique VH6 clones arising from 18 unique rearrangements. 2 of the 12 related clones were expressed with IgM, 2 with IgG, and 8 with IgA1. All the clones, including those expressin...

Abstract Immunotherapeutic approaches in cancer aim to booster T cell mediated immune responses. Tumor-specific cells recognize target via human leukocyte antigen (HLA) molecules before specific lysis is initiated. Malignant gliomas are known for their immunoevasive and immunosuppressive properties which impede effective tumor-specific responses, including clinical responses checkpoint inhibiti...

From the chemical evidence presented it appears that the toxicity associated with the somatic antigen of Type Z Sh. paradysenteriae may be attributed to an entity distinct from the known components of the complex. That this substance is neither the protein, the lipid, nor the carbohydrate component of the antigen is evident. Because of its lability to chemical manipulation, the nature of the to...

strongyloidiasis, caused by a nematode parasite so-called strongyloides stercoralis is one of the major human intestinal nematode infections. considering that stool examination for strongyloides larvae is not a sensitive method and immunodiagnostic methods are more applicable for this purpose, so the present study was conducted to compare the somatic (s) and excretory - secretory (es) antigens ...

When Epstein-Barr virus (EBV) negative cells (Raji) were treated with iododeoxyuridine, only the early antigen (EA) component was induced. There was no significant increase in EBV DNA and no virus particles were observed. Somatic-cell hybrids were prepared from the fusion of Raji and D98 cells (D98/Raji). When these cells were treated with iododeoxyuridine, early antigen EBV DNA, and virus part...

Immunization of animals with a liposomal capsular and major somatic antigen of a plague causative agent enhanced their protective effects against plague infection. Lipopolysaccharide incorporation into the lipid membrane resulted in an increased protective effect of antigen-containing liposomes, which may be related to the single delivery and interaction with immune-competent cells. Incorporati...

B lymphocytes undergo affinity maturation of their antigen receptors within germinal centers. These anatomical structures develop in secondary lymphoid organs from the clonal expansion of a few antigen-specific founder B cells, whose isolation and characterization are reported here. Human germinal center founder cells express the naive B cell markers surface IgM and IgD as well as the germinal ...

PURPOSE Most lymphomas of the ocular adnexa are primary extranodal non-Hodgkin's lymphomas of the B-cell type, with the most common lymphoma subtype being the extranodal marginal-zone B-cell lymphoma (EMZL). Analysis of somatic mutations in the variable (V) region of the Ig heavy (H)-chain gene segment suggests that EMZL development in other locations is dependent on antigen stimulation. The pu...

Broadly neutralizing antibodies (bNAbs) to HIV-1 can prevent infection and are therefore of great importance for HIV-1 vaccine design. Notably, bNAbs are highly somatically mutated and generated by a fraction of HIV-1-infected individuals several years after infection. Antibodies typically accumulate mutations in the complementarity determining region (CDR) loops, which usually contact the anti...