The edible blue-green alga (cyanobacterium), Suizenji-nori, contained 143.8+/-22.4 microg of vitamin B(12) per 100 g dry weight of the alga (mean+/-SE, n=4). A corrinoid compound was purified from the dried Suizenji-nori, and partially characterized. The silica gel 60 TLC and reversed-phase HPLC patterns of the purified corrinoid compound were not identical to those of true vitamin B(12), but t...

Three antioxidant food supplements were analysed for selenium (Se) and its species. Sample A main constituents were coenzyme Q10, selenium (as medical yeast), vitamin C and natural vitamin E. The product is used for maintaining health and strengthening physical and mental abilities, stimulating the immune system, inhibiting the development of atherosclerosis, strengthening a weakened heart. Sam...

Monodentate phosphoramidite ligands have been developed based on enantiopure 6,6'-dimethylbiphenols with axial chirality. These chiral ligands are easy to prepare and flexible for modifications. The fine-tuning capability of these ligands plays a significant role in achieving high enantioselectivity in the asymmetric hydroformylation of allyl cyanide and the conjugate addition of diethylzinc to...

O objetivo deste trabalho foi determinar a aplicabilidade de dois métodos enzimáticos um para a quantificação da glucose e outro da sacarose em soluções aquosas contendo quitosano. Os métodos analíticos foram validados e os principais parâmetros determinados como limite de detecção, intervalo de linearidade, precisão e exatidão. A incerteza global do método enzimático da glucose foi inferior a ...

Nine grain sorghum cultivars with a broad range of ethanol fermentation efficiencies were selected to characterize the changes in sorghum protein in digestibility, solubility, and microstructure during mashing and to relate those changes to ethanol fermentation quality of sorghum. Mashing reduced in vitro protein digestibility considerably, and a large amount of polymers cross-linked by disulfi...

The levels of 13 proteins were measured in six tear samples collected atraumatically at progressively increasing flow rate from nonstimulated (less than 0.5 microliter/min) to highly stimulated (greater than 50 microliters/min) in ten subjects. Tears were fractionated initially by size-exclusion high-performance liquid chromatography (SE-HPLC). Enzyme-linked immunosorbent assays and kinetic ass...

The tannin fractions isolated from hazelnuts, walnuts and almonds were characterised by colorimetric assays and by an SE-HPLC technique. The complexation of Cu(II) and Zn(II) was determined by the reaction with tetramethylmurexide, whereas for Fe(II), ferrozine was employed. The walnut tannins exhibited a significantly weaker reaction with the vanillin/HCl reagent than hazelnut and almond tanni...

A simultaneous extraction of Cr3+ and Cr6+ species in aqueous solution was developed. The extraction behaviors of ammonium pyrrolidinedithiocarbamate (APDC) for both Cr6+ and Cr3+ are carefully discussed in this report. X-ray diffraction and spectroscopic studies indicate that Cr6+ reacts with the ammonium pyrrolidinedithiocarbamate ligand to form two products, Cr(PDC)2(OPDC) and Cr(PDC)3, wher...

An analytical method was developed for the speciation of elemental selenium (Se(0)) in selenized yeasts by anion-exchange HPLC-ICP-MS after its chemical transformation into SeSO32− reaction with sodium sulfite. The presence Se(0) further confirmed single-particle ICP-MS. Indeed, Se nanoparticles, if present, are expected to be, at least partly, Se(0). X-ray photoelectron spectroscopy, a well-re...

BACKGROUND Preeclampsia is associated with a quantitative imbalance between lipid peroxide and an antioxidant coproduced in the placenta. To investigate our hypothesis that 2-hydroxyestradiol 17-sulfate (2-OH-ES) is the placental antioxidant during pregnancy, we developed an assay for 2-OH-ES in urine and studied samples from women with and without preeclampsia. METHODS The detection and meas...