نتایج جستجو برای: salmonella antibody igy and igg in egg yolk and serum respectively
تعداد نتایج: 21786645 فیلتر نتایج به سال:
White leghorn hens were immunized intramuscularly with formalin killed Klebsiella pneumoniae cells to generate Klebsiella pneumoniae antibodies (IgY) in egg yolk. Booster injections of increasing concentrations of antigen were given to raise the antibody level in egg yolk. Amount of protein varied from 0.87 7.4mg/ml in egg yolk throughout the immunization period. The antibodies were partially p...
Monoclonal antibodies (Mabs) were produced against chicken IgY purified from egg yolks. Purity of the IgY was established using immunoelectrophoresis and reducing and non-reducing polyacrylamide gel electrophoresis. Five stable hybrid clones were characterized and Mabs, all belonging to IgG1 isotype, were found to bind to the IgY heavy chain in Western blots. These clones did not cross-react wi...
An indirect sandwich ELISA that can detect as little as 8 ng of African horsesickness virus (AHSV) was developed. Viral antigen was captured from suspension using an immobilized monoclonal antibody specific for an epitope on VP7, a protein that is a major constituent of the virus core. Egg-yolk derived chicken IgY directed against AHSV (serotype 3) was used as the secondary antibody. Since IgY ...
background: cryptosporidium parvum is a parasitic protozoan that functions as important causative agent of diarrhea in human and animals. the host's immune response to surface antigens of c. parvum has been previously demonstrated. in this respect, the role of humoral immunity in the development of host protective immunity against this protozoon has been well demonstrated. methods: the effe...
Indirect enzyme linked immunosorbent assay was developed to measure specific antibody activity to outer membrane proteins in sera and egg yolk of chickens exposed to Salmonella Gallinarum infection and also following vaccination. The observed end point titers were used to formulate regression equation and the absorbance data obtained at a single serum dilution were converted directly to antibod...
attempts have been made to study the thermodynamic behavior of 1,3 butadiene purification columns with the aim of retrofitting those columns to more energy efficient separation schemes. 1,3 butadiene is purified in two columns in series through being separated from methyl acetylene and 1,2 butadiene in the first and second column respectively. comparisons have been made among different therm...
this research was conducted in two protect and destroy region in the middle zagros, in illam province. in order to identification of ecological species group and evaluate density of regeneration, effect of many factors such as protection, vegetation, physiographic factors, physical and chemical properties of soil in study locations were studied. to achieve these purpose number of 54 plots using...
Egg yolk antibodies, called IgY, were isolated from the egg yolks of chickens immunized with enterotoxigenic Escherichia coli (ETEC) 987P. Specific binding activity of IgY against ETEC 987P was found. Whole cell titers remained relatively high during the immunization period (up to 19 weeks). The IgY antibodies cross-reacted with other members of Enterobacteriaceae, Escherichia coli O157:H7, Sal...
Objective: The objective of the present investigation was to develop Chicken Egg Yolk antibodies as an alternative and affordable approach for diagnosis and treatment against dental caries in humans. Materials and Methods: Twenty four week old white leghorn chicken was immunized with formalin killed Streptococcus mutans (MTCC 890) to produce Egg Yolk antibodies (IgY). The amount of egg yolk ant...
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