Genomic mapping of DNA replication origins (ORIs) in mammals provides a powerful means for understanding the regulatory complexity of our genome. Here we combine a genome-wide approach to identify preferential sites of DNA replication initiation at 0.4% of the mouse genome with detailed molecular analysis at distinct classes of ORIs according to their location relative to the genes. Our study r...

State Machine Replication (SMR) is a fundamental technique for ensuring the dependability of critical services in modern internet-scale infrastructures. SMR alone does not protect from full crashes, and thus in practice it is employed together with secondary storage to ensure the durability of the data managed by these services. In this work we show that the classical durability enforcing mecha...

A detailed quantitative kinetic model for the polymerase chain reaction (PCR) is developed, which allows us to predict the probability of replication of a DNA molecule in terms of the physical parameters involved in the system. The important issue of the determination of the number of PCR cycles during which this probability can be considered to be a constant is solved within the framework of t...

Distributed Shared Memory (DSM) systems implemented on a network of workstations (NOW) have become a convenient alternative to shared memory archi-tectures to execute long running parallel applications. However, such architectures are susceptible to experience failures. This paper presents the design and implementation of a recoverable DSM (RDSM) based on a backward error recovery (BER) mechani...

UNLABELLED Translational readthrough--suppression of termination at a stop codon--is exploited in the replication cycles of several viruses and represents a potential target for antiviral intervention. In the gammaretroviruses, typified by Moloney murine leukemia virus (MuLV), gag and pol are in the same reading frame, separated by a UAG stop codon, and termination codon readthrough is required...