نتایج جستجو برای: recombinant plasmids

تعداد نتایج: 124414  

Chicken infectious bronchitis virus (IBV) causes severe respiratory and renal dysfunction in chickens. In the present study, we used reverse transcription-polymerase chain reaction (RT-PCR) to amplify the nucleocapsid (N) protein gene of strain Massachusetts (Mass) H120 of IBV that is commonly used in the vaccine production in Iran. The PCR product with the expected size of 1.2 kb was cloned in...

Journal: :modares journal of medical sciences: pathobiology 2012
samaneh hossainzadeh fatemeh fotouhi behrokh farahmand maryam saleh atena yousefi

objective: influenza virus a (h1n1) is an important subtype of the influenza respiratory viruses, which has important worldwide implications. hemagglutinin (ha), an important viral antigen, is responsible for binding to human cell receptors leading to an onset of the disease process. considering the critical role of viral attachment, this study focuses on the extraction and cloning of ha and it...

Abbas Ghaderi, Amin Ramezani , Elham Mahmoudi Maymand, Fatemeh Sadat Toghraie, Mahsa Yazdanpanah-Samani, Seyedeh Masoumeh Sharifzadeh,

Background: The critical role of interleukin-7 (IL-7) in homeostatic proliferation and T cell survival has made it a promising cytokine for the treatment of various clinical conditions, especially those associated with lymphopenia. Methods: In the present study we expressed recombinant human interleukin-7 (rhIL-7) in Chinese hamster ovary (CHO)-K1 cells. CHO-K1 cells were stably transfected ...

کی, مریم, حجتی, زهره , حیدری, مریم ,

Background: Interferon beta is one of the most important members of group I interferons and is the main drug for multiple sclerosis treatment. Interferon beta has short half life and this compels patients to make frequent use of medicine. According to its clinical usage there is broad effort to improve translation level and protein production. There are several important factors which effect pr...

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