Polyhydroxybutyrate (PHB) is a bioplastic that can be produced in transgenic plants by the coexpression of three bacterial genes for its biosynthesis. PHB yields from plants have been constrained by the negative impacts on plant health that result from diversion of resources into PHB production; thus, we employed an ecdysone analogue-based system for induced gene expression. We characterized 49...

The production of polyhydroxybutyrate (PHB) by Bacillus sp. is most often growth associated and is influenced by various physico-chemical parameters. Imbalanced nutrient conditions were often found to result in sporulation and low PHB production in Bacillus sp. In the present investigation, Bacillus mycoides DFC1 strain isolated from garden soil was studied for PHB production in glucose–peptone...

Polyhydroxybutyrate (PHB) is a common carbon storage polymer among heterotrophic bacteria. It is also accumulated in some photoautotrophic cyanobacteria; however, the knowledge of how PHB accumulation is regulated in this group is limited. PHB synthesis in Synechocystis sp. PCC 6803 is initiated once macronutrients like phosphorus or nitrogen are limiting. We have previously reported a mutation...

Polymerase is a key enzyme involved in the biosynthesis of polyhydroxybutyrate (PHB), a well-known bacterial biodegradable polyester. In this study, we have established an in vivo assay system to analyze mutational effects of Ralstonia eutropha polymerase (termed PhbCRe) on the level of PHB accumulation in recombinant strains of Escherichia coli. This in vitro evolution system consists of polym...

Polymerase is a central enzyme involved in the biosynthesis of polyhydroxybutyrate (PHB), a well-known bacterial biodegradable polyester. In this study, we have established an in vivo assay system to analyze mutational effects of Ralstonia eutropha polymerase (termed PhbC(Re)) on the level of PHB accumulation in recombinant strains of Escherichia coli. This in vitro evolution system consists of...

The Cassava starch manufacturing wastewater (CSW) was used as a substrate to produce polyhydroxybutyrate (PHB) by Cupriavidus sp. KKU38. The acidogenic fermentation process of CSW was first conducted to obtain volatile fatty acids (VFAs), which are more efficient in PHB production than raw CSW. The effect on substrate concentration and nutrients, i.e. nitrogen and phosphorus concentrations, by ...

Fibrous scaffolds based on biodegradable and biocompatible polyhydroxybutyrate (PHB) were produced by electrospinning. The coated a thin solid film of polypyrrole, PPyI, deposited plasma polymerization technique. FTIR Raman spectroscopy results confirm the presence PPyI PHB fibers, in can be show signal NH2 groups characteristic so broad band due to fluorescence from coating. morphology was cha...

The intended circular economy for plastics envisages that they will be partially replaced by bio-based polymers in the future. In this work, natural polyester polyhydroxybutyrate (PHB) was produced Azohydromonas lata using cheese whey (CW) as a low-cost substrate. Initially, CW evaluated sole carbon source PHB production; it found to efficient and comparable production with pure sugars, such sa...