Yeast histone mRNAs are polyadenylated, yet factors such as Rrp6p and Trf4p, required for the 3'-end processing of non-polyadenylated RNAs, contribute to the cell cycle regulation of these transcripts. Here, we investigated the role of other known 3'-end processing/transcription termination factors of non-polyadenylated RNA in the biogenesis of histone mRNAs, specifically the Nab3p/Nrd1p/Sen1p ...

It is a challenging task to predict with high reliability whether plant genomic sequences contain a polyadenylation (polyA) site or not. In this paper, we solve the task by means of a systematic machine-learning procedure applied on a dataset of 1000 Arabidopsis thaliana sequences flanking polyA sites. Our procedure consists of three steps. In the first step, we extract informative features fro...

Duodenogastric reflux of bile acids and lysolecithin in the course of a standard test meal was measured in normal people and in patients with duodenal ulcer before operation and more than one year after highly selective vagotomy, Polya partial gastrectomy, truncal vagotomy and pyloroplasty, and truncal vagotomy and gastrojejunostomy. Before operation, duodenal ulcer patients had significantly h...

Results The median age overall was 17.1(13-21) years. 28.9% (46) of individuals had ERA, of which 69.6% (39) were male. Mean duration of disease since diagnosis was 7.3 years (SD=+/-4.9). Of the other JIA subtypes; 21.4% (34), 17.6% (28) and 11.9% (19) had Extended Oligoarticular Arthritis (EOA), Polyarticular Rheumatoid Factor Positive (JIA RF+) and Polyarticular Rheumatoid Factor Negative (JI...

Many classical methods in finite population sampling have been developed to incorporate some fairly standard types of prior information about the population. When the prior information does not fall in the standard categories it can be difficult to find procedures which reflect the information and have good frequentist properties. Recently methods based on the empirical likelihood were consider...

Let f(z) be any transcendental entire function. Let r, denote the absolute value of the zero z. of f '(z) which is nearest to the origin. Alander, Erdö's and Renyi, and Polya have investigated the relation between r, and the growth of the function /(z). Let s, denote the ... fz largest disk about the origin where / (z) is univalent. Boas, Levinson, and Polya have obtained some relations between...

Alternative polyadenylation is a key regulatory process which affects the 3' end formation of variants of the same transcription unit, thus altering gene expression pattern, and transcripts' cellular behaviour and characteristics. The common methodology for computational analysis of alternative polyadenylation signal utilization is based on EST data, specifically on PolyA/PolyT tailed ESTs. Stu...

BACKGROUND The starting material for RNA sequencing (RNA-seq) studies is usually total RNA or polyA+ RNA. Both forms of RNA represent heterogeneous pools of RNA molecules at different levels of maturation and processing. Such heterogeneity, in addition to the biases associated with polyA+ purification steps, may influence the analysis, sensitivity and the interpretation of RNA-seq data. We hypo...

MOTIVATION Next-generation RNA sequencing offers an opportunity to investigate transcriptome in an unprecedented scale. Recent studies have revealed widespread alternative polyadenylation (polyA) in eukaryotes, leading to various mRNA isoforms differing in their 3' untranslated regions (3'UTR), through which, the stability, localization and translation of mRNA can be regulated. However, very fe...

Immunization with defined tumor antigens is currently limited to a small number of cancers where candidates for tumor rejection antigens have been identified. In this study we investigated whether pulsing dendritic cells (DC) with tumor-derived RNA is an effective way to induce CTL and tumor immunity. DC pulsed with in vitro synthesized chicken ovalbumin (OVA) RNA were more effective than OVA p...