The objective of this research was to investigate the influence of the over-expression of recombinant interferon-g during high cell density cultivation on cellular characteristics of recombinant E. coli. Batch and fed-batch culture techniques were employed to grow Escherichia coli BL21 for production of human gamma-interferon in pET expression system. Final cell densities in batch and fed-batch...

A total of 145 Escherichia coli isolates originated from clinically-healthy pigs were analyzed for plasmid profiles. All but one isolate (n=144) carried one to nine plasmids with size ranging from 0.7-16.2 Mda. Fifty-six plasmid profiles were defined based on sizes and numbers of plasmids in each strain, of which the most common patterns were 15.8, 4, 3.3, 1.7, 1.3 Mda and 16.2, 15.8, 4, 3.3, 2...

Resistance to different antibiotics was found in 26 of the 30 strains analyzed, more than 70% of the strains analyzed were resistant to carbenicillin and ampicillin and a significant correlation was found between the resistance to both antibiotics. Plasmids were found in 80% of the strains analyzed, and 11 different plasmid profiles were observed. The most common profile obtained had only a 21....

Objective(s):Urinary tract infection (UTI) caused by bacteria is one of the most frequent infections in human population. Inappropriate use of antibiotics, often leads to appearance of drug resistance in bacteria. However, use of probiotic bacteria has been suggested as a partial replacement. This study was aimed to assess the antagonistic effects of Lactobacillus standard strains against bacte...

Strains of Salmonella muenster displaying identical antibiotic susceptibility and bacteriophage reaction patterns were found to differ in their complements of plasmid DNA. Plasmid profiles were used to separate 40 isolates from diverse sources into four distinct groups, one of which contained 30 strains without plasmids.

Enteropathogenic Escherichia coli (EPEC) is a leading cause of diarrhea among infants in developing countries. A total of 38 EPEC isolates, obtained from diarrhea patients of Hospital Miri, Sarawak, were investigated through plasmid profile, antibiotic resistance and randomly amplified polymorphic DNA (RAPD) analysis. From the 8 types of antibiotics used, all isolates were 100% resistant to fur...

In at least three Bacillus thuringiensis subspecies, multiple protoxin genes are confined to just a few of the many plasmids with two or more on one of > 100 mDa and a particular gene, cryIA(b), on a 40-50 mDa plasmid. The latter is unstable but can be maintained in the population by cell mating. Cells which had lost this plasmid compensated by increasing transcription of the remaining protoxin...

A total of 110 clinical isolates of Clostridium difficile were analyzed by agarose gel electrophoresis by using both bacterial restriction endonuclease analysis (REA) and plasmid profiles. A total of 72 isolates were divided into 12 groups according to their REA patterns. Some 38 isolates exhibited unique patterns. Pattern A occurred in 20% of isolates. Isolates with patterns B, E, and G were c...