Objective. Using the data obtained in Perm Region as an example, to identify effectiveness of polymerase chain reaction (PCR) for diagnosis Human Monocytic Ehrlichiosis (HME) at different periods from onset disease, and determine role HME structure infections transmitted by ixodic ticks using PCR enzyme-linked immunosorbent assay (ELISA)
 Materials methods. A thorough clinical epidemiologi...

The development of a rhinovirus (RV)-RNA-specific reverse transcription (RT)-PCR assay is complicated by the close homology between the RV and enterovirus (EV) genomes in the highly conserved 5'-noncoding region, which is chosen for primer design in most RT-PCR assays. We have developed a sensitive, rapid, and RV-specific nested RT-PCR assay and have used it to test nasopharyngeal aspirates fro...

BACKGROUND Current laboratory and radiological methods for diagnosis of invasive aspergillosis (IA) lack sensitivity and specificity. METHODS We prospectively evaluated the diagnostic value of twice-weekly screening for circulating Aspergillus fumigatus and A. flavus DNA with a polymerase chain reaction-enzyme-linked immunosorbent assay (PCR-ELISA). RESULTS Among the 201 adult patients with...

There is a need for a rapid, uncomplicated, and inexpensive test for Chlamydia trachomatis infection in women. We evaluated the ability of a 6-min enzyme-linked immunosorbent assay (ELISA) that requires no laboratory equipment (IgA Rapid SeroTest; Savyon Diagnostics) to detect C. trachomatis immunoglobulin A (IgA) in the endocervices of 167 inner-city pregnant women and compared the results wit...

Canine visceral leishmaniasis (CVL) is endemic in numerous Brazilian regions. The greatest difficulty in controlling the disease is the diagnostic limitation. In the present study, the most common tests employed for visceral leishmaniasis diagnosis were compared: immunofluorescence antibody test (IFAT), immunoenzymatic assay (ELISA), direct parasitological examination and polymerase chain react...

To standardize a PCR assay for the detection of parvovirus B19 DNA in serum samples three different sample treatments were evaluated on the basis of the efficiency of recovery, reproducibility, convenience of sample handling, and presence of PCR inhibitors. Moreover, the presence of an internal standard competitor as the working reagent at one defined concentration in a competitive PCR-ELISA ha...

AIMS To evaluate the sensitivity and specificity of serological, immunohistochemical, and molecular methods in the diagnosis of post kala-azar dermal leishmaniasis (PKDL). METHODS Twenty five patients with confirmed PKDL and 25 controls were included in the study. G2D10, a monoclonal antibody against Leishmania, was used for the immunohistochemical (IHC) staining of lesion sections to visuali...

methods this experimental study was conducted on iranian children communities from may to november 2014. forty stool samples were obtained from laboratories and investigated for heat-labile toxin (lt). specific primers were designed and the dig -labeled pcr products were bounded to streptavidin-coated wells of a microtiter plate and detected by anti-dig-peroxidase conjugate. an internal biotin-...

Antigen-coated plate enzyme-linked immunosorbent assay (ACP-ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) were used to detect the presence seed transmissibility of bean common mosaic virus-blackeye cowpea (BCMV-BICM) in farm- retained lots obtained from 46 locations, including markets farms major growing areas Ashanti Brong Ahafo regions Ghana. In growout tests, virus symp...

AIM To evaluate a newly developed aspergillus mitochondrial gene PCR-ELISA assay for the early diagnosis of invasive pulmonary aspergillosis (IPA) in neutropenic patients. METHODS The aspergillus mitochondrial gene was chosen for the amplification target for use with a solution hybridisation assay with colorimetric end stage detection in microtitre plate format (PCR-ELISA). The study group co...