We investigated the interaction of Rice grassy stunt tenuivirus (RGSV) nonstructural protein p5, a protein of 22 kDa encoded on vRNA 5, with all 12 RGSV proteins by using a GAL4 transcription activator-based yeast two-hybrid system. The p5 protein interacted only with itself and not with any other viral protein; the interacting domains were localized within the N-terminal 96 amino acids of p5. ...

The synthesis of sequence-specific 2'-5'-oligonucleotides and analogues of 2'-5' linked oligoadenylates containing 8-substituted adenosine derivatives [8-hydroxypropyladenosine (AHPr) and 8-hydroxyadenosine (AOH)] is reported. The reaction of 5'-phosphoroimidazolidate of 8-substituted adenosines under conditions of lead ion catalyst did not give the corresponding 2'-5' oligoadenylates containin...

4 With respect to a class C of graphs, a graph G ∈ C is said to be k-critical if every proper 5 subgraph of G belonging to C is k−1 colorable. We construct an infinite set of k-critical P56 free graphs for every k ≥ 5. We also prove that there are exactly eight 5-critical {P5, C5}-free 7 graphs. 8

RNA interference (RNAi) is a process that involves the delivery of small single stranded RNA molecules into mammalian cells, resulting in the sequence-specific cleavage of complementary mRNA and the silencing of specific gene expression. These small RNA molecules called small interfering RNAs (siRNAs) are the focus of intense research due to their potential therapeutic uses in various disease p...

List accessing problem has been studied as a problem of significant theoretical and practical interest in the context of linear search. Various list accessing algorithms have been proposed in the literature and their performances have been analyzed theoretically and experimentally. Move-To-Front (MTF), Transpose (TRANS) and Frequency Count (FC) are the three primitive and widely used list acces...

Although the vertebrate embryonic midline plays a critical role in determining the left/right asymmetric development of multiple organs, few genes expressed in the midline are known to function specifically in establishing laterality patterning. Here we show that a gene encoding protein disulfide isomerase P5 (PDI-P5) is expressed at high levels in the organizer and axial mesoderm and is requir...

The use of peptide libraries for the identification and characterization of T cell antigen peptide epitopes and mimotopes has been hampered by the need to form complexes between the peptides and an appropriate MHC molecule in order to construct a complete T cell ligand. We have developed a baculovirus-based peptide library method in which the sequence encoding the peptide is embedded within the...

Thirteen monoclonal antibodies to ppp(A2'p5')nA oligonucleotides have been produced by fusing Y3 myeloma cells with spleen cells of rat hyperimmunized with A2'p5'A succinyl albumin as immunogen. 125I-labeled A2'p5'A succinyl tyrosine methyl ester was used as a labeled probe. Antibodies were detected by their ability to bind the labeled analog and were selected for their affinity for A2'p5'A. Th...

Chemical modification of p5’A2’(p5’A2’),p5’A by a periodate oxidation/Schiff base formation/borohydride reduction cycle gave a series of 2-5A analogues in which the ribose of the 2’-terminal nucleotide was transformed to an N-substituted morpholine (azahexapyranose). 2’,5’-Oligoriboadenylate 5’-monophosphates bearing this modification were 5-10 times more potent as antagonists of the action of ...