The nucleoprotein genes of influenza virus A/Netherlands/018/94 (H3N2) and influenza virus B/Harbin/7/94 were cloned into the bacterial expression vector pMalC to yield highly purified recombinant influenza virus A and B nucleoproteins. With these recombinant influenza nucleoproteins, enzyme-linked immunosorbent assays (ELISAs) were developed for the detection of influenza virus A- and B-specif...

background and aims: influenza virus nucleoprotein (np) has the capacity to be used as subunit vaccine, but little is known about the impact of different cultures on its structure. in the present study we aimed to evaluate and compare the isoelectric focusing (ief) property of extracted viral nucleoproteins derived from madin darby canine kidney (mdck) cell line and embryonated chicken eggs (ec...

Evolution drives changes in a protein's sequence over time. The extent to which these changes in sequence lead to shifts in the underlying preference for each amino acid at each site is an important question with implications for comparative sequence-analysis methods, such as molecular phylogenetics. To quantify the extent that site-specific amino acid preferences shift during evolution, we per...

The present work was undertaken to study the distribution of yeast nucleoprotein in cell fractions after centrifugal separation, and to define conditions of extraction and purification in order to obtain the nucleoprotein in a relatively undegraded state. Bakers’ yeast (Xaccharomyces cerevisiae) is the classical source of ribonucleic acid (RNA), but relatively little work has been reported on t...