L. Z. Shi, Q. Zhu, T. Wu, M. L. Duquette, V. Gomez, C. Chandsawangbhuwana, M. S. Harsono, N. Hyun, N. Baker, J. Nascimento, Z. You, E. B. Botvinick and M. W. Berns Institute of Engineering in Medicine, University of California, San Diego, 9500 Gilman Dr. SERF 295, MC 0435 La Jolla, CA 92093, USA Beckman Laser Institute, University of California, Irvine, 1002 Health Sciences Road, Irvine, CA 926...

Stress fibers are actomyosin-based bundles whose structural and contractile properties underlie numerous cellular processes including adhesion, motility and mechanosensing. Recent advances in high-resolution live-cell imaging and single-cell force measurement have dramatically sharpened our understanding of the assembly, connectivity, and evolution of various specialized stress fiber subpopulat...

The ability of a cell to distribute contractile stresses across the extracellular matrix in a spatially heterogeneous fashion underlies many cellular behaviors, including motility and tissue assembly. Here we investigate the biophysical basis of this phenomenon by using femtosecond laser nanosurgery to measure the viscoelastic recoil and cell-shape contributions of contractile stress fibers (SF...

For genomic integrity to be maintained, the cell cycle and DNA damage responses must be linked. Cdt1, a G1-specific cell-cycle factor, is targeted for proteolysis by the Cul4-Ddb1(Cdt2) ubiquitin ligase following DNA damage. Using a laser nanosurgery microscope to generate spatially restricted DNA damage within the living cell nucleus, we show that Cdt1 is recruited onto damaged sites in G1 pha...

In this article, we propose a molecular communication system to localize an abnormality in diffusion-based medium. We consider general setup perform joint sensing, communication, and localization. This consists of three types devices, each for different task: mobile sensors navigation molecule releasing (for communication), fusion centers (FCs) sampling, amplifying, forwarding the signal, gatew...

The Golgi complex is the central organelle of the secretory pathway. It undergoes dynamic changes during the cell cycle, but how it acquires and maintains its complex structure is unclear. To address this question, we have used laser nanosurgery to deplete BSC1 cells of the Golgi complex and have monitored its biogenesis by quantitative time-lapse microscopy and correlative electron microscopy....

While is widely acknowledged that nonmuscle myosin II (NMMII) enables stress fibers (SFs) to generate traction forces against the extracellular matrix, little is known about how specific NMMII isoforms and functional domains contribute to SF mechanics. Here we combine biophotonic and genetic approaches to address these open questions. First, we suppress the NMMII isoforms MIIA and MIIB and appl...

Ultrafast laser breakdown of wide bandgap dielectrics is today a key for major technologies ranging from 3D material processing in optical materials to nanosurgery. However, contradiction persists between the strongly nonlinear character energy absorption and robustness processes changes bandgap/wavelength ratio depending on applications. While various bandgaps have been studied, we concentrate...

Plasmonic nanoparticles can lead to extreme confinement of the light in the near field. This unique ability of plasmonic nanoparticles can be used to generate nanobubbles in liquid. In this work, we demonstrate with single-particle monitoring that 100 nm gold nanoparticles (AuNPs) irradiated by off-resonance femtosecond (fs) laser in the tissue therapeutic optical window (λ = 800 nm), can act a...