BACKGROUND Improved chemical hazard management such as REACH policy objective as well as drug ADMETOX prediction, while limiting the extent of animal testing, requires the development of increasingly high throughput as well as highly pertinent in vitro toxicity assays. METHODOLOGY This report describes a new in vitro method for toxicity testing, combining cell-based assays in nanodrop Cell-on...

The assessment of RNA integrity is a critical first step in obtaining meaningful gene expression data. Working with low-quality RNA may strongly compromise the experimental results of downstream applications which are often labour-intensive, time-consuming, and highly expensive. Using intact RNA is a key element for the successful application of modern molecular biological methods, like qRT-PCR...

PM2 (Corticoviridae) is a dsDNA bacteriophage which contains a lipid membrane beneath its icosahedral capsid. In this respect it resembles bacteriophage PRD1 (Tectiviridae), although it is not known whether the similarity extends to the detailed molecular architecture of the virus, for instance the fold of the major coat protein P2. Structural analysis of PM2 has been initiated and virus-derive...

PURPOSE To evaluate the expression of microRNAs in tissue samples from patients with bladder cancer and to compare it with healthy adjacent tissue samples as controls. MATERIALS AND METHODS Thirty five tissue samples from patients with newly diagnosed untreated bladder transitional cell carcinoma and 35 adjacent normal urothelium were collected during 2013 to 2014. TRIzol reagent was used to ...

Detection of pathogenic bacteria in food is most important for food safety and quality control, and the critical step it chooses the rapid, sensitive and more economical method to extract DNA to produce high quality and decrease the timeconsuming of measuring. Extraction of nucleic acids is the first step in most molecular biology studies and in all recombinant DNA techniques, but the difficult...

Sequencing. Genomic DNA was extracted from peripheral blood using standard procedures and its quality was monitored using a Nanodrop spectrophotometer. DNA was amplified for CDKN2A, CDK4, MC1R, TP53 and MITF as described previously [13, S1]. PCR products were subjected to automatic sequencing by ABI PRISM 310 genetic analyzer (Applied Biosystems). Microsatellite analysis. The primers used for m...

BACKGROUND Sample processing is a crucial step for all types of genomic studies. A major challenge for researchers is to understand and predict how RNA quality affects the identification of transcriptional differences (by introducing either false-positive or false-negative errors). Nanotechnologies help improve the quality and quantity control for gene expression studies. PATIENTS AND METHODS...

Pseudomonas aeruginosa (PA) infections result in significant morbidity and mortality in hosts with compromised immune systems, such as patients with leukemia, severe burn wounds, or organ transplants. In patients at high-risk for developing PA bloodstream infections, the gastrointestinal (GI) tract is the main reservoir for colonization, but the mechanisms by which PA transitions from an asympt...

methods mycobacterium tuberculosis standard strain crbip7.11 was cultured at 37°c in loewenstein johnson (lj) media for 3-4 weeks. to confirm the species, standard microbiological and biochemical tests were performed. after preparation of membrane vesicles, the amount of protein in membrane vesicles was measured by sds-page and nanodrop. to analyze the integrity and morphology of extracellular ...

Major histocompatibility complex (MHC) molecules are heterodimeric cell-surface receptors that play a crucial role in the cellular immune response by presenting epitope peptides to T-cell antigen receptors (TCR). Although the structural basis of the peptide-MHC binding mechanism is becoming better understood, it is still difficult to predict a binding mode for an MHC of unknown structure. There...