Biodiesel production from microalgae depends on algal biomass through autotrophic cultivation, and it has a lot of potential as feasible alternative source energy. The lethal mutagenic effects ethyl methanesulphonate (EMS) are investigated in the present study organism Chlorella pyrenoidosa for generation paler mutants. C. wild-type strain was treated with 0.1, 0.2, 0.5 M EMS doses colonies tha...

ABSTRACT Germline sexual fate has long been believed to be determined by the somatic environment, but this idea is challenged recent studies of foxl3 mutants in medaka. Here, we demonstrate that tilapia germline antagonistic interaction dmrt1 and foxl3, which are transcriptionally repressed male female germ cells, respectively. Loss rescued cell sex reversal foxl3?7/?7 XX fish, loss partially n...

Leptospirosis is a global zoonosis caused by pathogenic bacteria of the genus Leptospira. The application CRISPR/Cas9 system has facilitated generation mutants and subsequent evaluation phenotypes. Since DNA breaks induced RNA-guided Cas9 nuclease are lethal to Leptospira, different methodologies were implemented overcome this limitation. Initially, CRISPR interference (CRISPRi) was employed cr...

herpes simplex virus 1 (hsv-1) unspliced 8.3 latency associated transcript (lat), which located in the long repeat sequences, has been shown to contain at least 16 open reading frames (orf: a-p). one of these orf, orf p, maps almost entirely antisense to hsv-1 neurovirulence gene, icp34.5. both orf p and icp34.5 are located in the long repeat and are antisense overlapping genes. therefore, in o...

BACKGROUND: Lots of plants that are used in Southeast Asian and Indonesian cuisine have antibacterial properties. AIM: This study aimed to find out the effectiveness lemon basil (Ocimum citriodorum) key lime (Citrus aurantifolia S.) extract combination on Streptococcus mutants’ inhibition. METHODS: Lemon Basil Key Lime were extracted combined with several following concentration, respectively, ...

The biological process of anther development is very complex. It remains largely unclear how the cinnamoyl–CoA reductase (CCR) encoding genes function in regulation plants. Here, we establish that CCR family gene OsCCR18 essential for maintaining male fertility rice. transcripts were greatly abundant panicles at S4 and S5 developmental stages subcellular localization proteins was nucleus knocko...

in this study, mutant forms of bacillus thuringiensis spp. israelensis (h14) were produced. these mutants were identified when the cells were cultured on chloramphenicol plates and stained with crystal violet. protoplasts of the mutants were isolated by enzymatic digestion (lysozyme) of the cell walls at the presence of an osmotic stabilizer. the protoplasts were induced to fuse to each other i...