The effect of hypothermia on the in vivo pharmacokinetics of midazolam was evaluated, with a focus on altered metabolism in the liver and binding to serum proteins. Rat primary hepatocytes were incubated with midazolam (which is metabolized mainly by CYP3A2) at 37, 32 or 28 °C. The Michaelis-Menten constant (Km) and maximum velocity (Vmax) of midazolam were estimated using the Michaelis-Menten ...

The main objective of this study was to purify and characterize an esterase from Shewanella sp F88. The enzyme was purified 41-fold and an overall yield of 21 %, using a two-step procedure, including ammonium sulfate precipitation and Q-sepharore chromatography. Molecular weight of the enzyme was 62.3 kDa according to SDS-PAGE data. The enzyme showed an optimum activity at pH 6.5 and 58 ˚C. Evo...

We present a model of an ATP-fueled molecular machine which push a polymer through a pore channel. The machine acts between two levels (workingwaiting), and the working one remains active for a fixed time giving a constant force. The machine activation rate can be put in relationship with the available ATP concentration in the solution, which gives the necessary energy supply. The translocation...

Calcium transport by sarcotubular vesicles (fragmented sarcoplasmic reticulum) from skeletal muscle of rats, rabbits, and humans follows Michaelis-Menten kinetics when studied in systems containing oxalate and low ratios of calcium to vesicle protein. Under these conditions different preparations of vesicles are characterized by their calcium concentrating ability, i.e. the constant level to wh...

The basis of enzyme kinetic modelling was established during the early 1900’s when the work of Leonor Michaelis and Maud Menten produced a pseudo-steady state equation linking enzymatic catalytic rate to substrate concentration (Michaelis & Menten, 1913). Building from the Michaelis-Menten equation, other equations used to describe the effects of modifiers of enzymatic activity were developed b...

in this research, an enzyme modified electrode has been produced during the electropolymerization of aniline through incorporation of sulfite oxidase into a conducting polymer. then the bioelectrochemical response of resulted sulfite biosensor was investigated at different experimental conditions. study of  the stability of the resulted sulfite biosensor revealed that formation of a passive fil...

In most of the real life problems, we encounter with nonlinear differential equations. Problems are made more understandable by modeling them these this way, it becomes easier to interpret problems and reach results. 1913, basic enzymatic reaction model introduced Michaelis Menten describe enzyme processes is an example equation. This one simplest best-known approaches mechanisms used enzyme-ca...

catalytic isomerization of glucose to fructose by immobilized glucose isomerase in an aqueous-ethanol medium was studied. using michaelis-menten equation and haldane relationship, the main kinetic parameters were determined: kmf, vmf, kmr, vmr. by comparing the aqueous-ethanol medium with an aqueous solution, the measured values of kmr and kmf were increased in the aqueous-ethanol solution, whi...