نتایج جستجو برای: isolation mitochondria
تعداد نتایج: 176965 فیلتر نتایج به سال:
Recently, our laboratory reported the detection of small amounts of DNA in lamb heart mitochondria (1, 2). Experimental evidence indicated that this DNA was not merely a contaminant, but fulfilled a biologically active role in mitochondrial protein synthesis (2, 3). This was established by the observation that a DNA-dependent RNA polymerase was present in the mitochondria (2, 4, 5). These obser...
Mitochondria isolated from normal mammary glands taken from lactating and nonlactating C3H mice were compared with those isolated from mammary adenocarcinomas. Functional mitochondria could be obtained from the normal mammary glands only when milk was not present in the tissue prior to homogenization and when bovine serum albumin was added to the isolation medium. Albumin also protected tumor m...
Mitochondria isolated from normal mammary glands taken from lactating and nonlactating C3H mice were compared with those isolated from mammary adenocarcinomas. Functional mitochondria could be obtained from the normal mammary glands only when milk was not present in the tissue prior to homogenization and when bovine serum albumin was added to the isolation medium. Albumin also protected tumor m...
(1) Activation of rat liver mitochondrial functions following glucagon treatment was demonstrated in mitochondria that had not been isolated by the conventional technique of differential centrifugation and washing in sucrose solutions. Crude liver homogenates in 0.3 M-sucrose or 0.15 M-KCI prepared from rats treated with glucagon showed stimulation of State-3 and uncoupled respiration, carboxyl...
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