Sequential injection immunoassay systems for environmental measurements based on the selective immunoreaction between antigen and antibody were described. A sequential injection analysis (SIA) technique is suitable to be applied for the procedure of enzyme-linked immunosorbent assay (ELISA), because the washing and the addition of reagent solutions can be automated by using a computer-controll...

A novel magnetic fluorescent encoded nanoimmunoassay system for multicomponent detection and separation of the subtrace pathogenic DNA (hepatitis B virus surface gene, HBV; hepatitis A virus poly the protein gene, HAV) was established based on new type of magnetic fluorescent encoded nanoparticles and sandwich immunoassay principle. This method combines multifunctional nanoparticles, immunoassa...

Screening for benzodiazepines is routinely performed by immunochemical analysis such as enzyme immunoassay. However, the wide range of therapeutic and toxic concentrations of the different benzodiazepines and their extensive metabolisation and conjugation hamper the design of an immunoassay protocol that can detect all benzodiazepines and their corresponding urinary metabolites. Only a few inve...

In evaluating the EMIT system for vancomycin used in the Cobas Bio centrifugal analyzer, we found two potential problems, each of which may have important clinical ramifications. First, precision, though acceptable in concentrations up to 30 mg/L, was marginal in the range above 30 mg/L. Second, when EMIT values were compared with those by fluorescent polarization immunoassay (TDx), we found a ...

Evaluation of Cyclosporin A (CyA) blood concentration is imperative in solid organ transplantation in order to achieve maximal immunosuppression with the least side effects. We compared the results of whole blood concentrations of CyA in 50 blood samples simultaneously evaluated by the fluorescent polarization immune assay (TDx) and the enzymatic competitive immune assay (EMIT 2000). There was ...

A powerful enzymatic mimetic has been fabricated by employing graphene oxide (GO) nanocolloids to disperse conductive carbon supports of hydrophobic carbon nanotubes (CNTs) before and after the loading of Pt nanocatalysts. The resulting GOCNT-Pt nanocomposites could present improved aqueous dispersion stability and Pt spatial distribution. Unexpectedly, they could show greatly enhanced peroxida...

Introduction: The diagnosis of pregnancy from forensic bloodstains can be useful in cases infanticide, criminal abortions and missing person identification. Objective: This research illustrated the use a rapid, precise, tremendously responsive enzyme immunoassay kit designed for medical usage, which is put to good our lab qualitative HCG detection blood stains has proven tool Methods: concentra...

background: enzyme linked immunosorbent assay (elisa) has been described as an alternative to radioimmunoassay for the mammalian and nonmammalian steroids detection. in this study, a simple and rapid elisa is described and validated for 4-pregnen-3,20, dione (progesterone). materials and methods: a general procedure for preparation of the acetylcholinesterase labelled steroid is described which...

We have modified the EMIT (Syva) serum digoxin assay for use with a mini-disc centrifugal analyzer. A minicomputer is used to control data acquisition, calculations, and readout for 14 cuvettes simultaneously. Thus, it is not necessary for time each individual assay manually or to plot calibration curves on graph paper. We use incubation times and dilutions similar to those used for the manual ...