Background: Radiotherapy is regarded as a standard treatment modality in breast cancer (BC). Radiation causes cellular damage both in cancer and normal cells by inducing DNA-damage and chromosomal aberrations (CA). Different agents were used for ameliorating effects of radiation, mainly antioxidants such as melatonin. Melatonin shows oncostatic properties on human BC. The aim of this study was ...

Methylsensitive comet electrophoresis is based on the assessment of level DNA migration from individual lysed cells after treatment with methylsensitive restriction enzymes. Using model human lymphocytes, optimal combination intensity and time was selected a new approach for evaluating relative methylation proposed. It established that in T98G culture, which are actively proliferating, higher t...

Significant cytotoxic lymphocyte-induced DNA fragmentation does not occur in four human target cells lysed by human natural killer lymphocytes, killer lymphocytes, or cytotoxic T lymphocytes. These results contrast with the extensive DNA fragmentation that occurs in murine target cells lysed by the analogous effector lymphocytes. Thus, DNA fragmentation, or the lack thereof, represents a specie...

Background: Boron and gadolinium are among the nuclides that hold a unique property of being a neutron capture therapy agent. Neutron beams have often a considerable portion of gamma rays with fast neutrons. Gamma rays, as beam contaminants, can cause considerable damage to normal tissues even if such tissues do contain high boron concentrations. Materials and Methods: The modification of radio...

introduction: bleomycin sulfate is a dna damaging agent used in cancer chemotherapy. the effect of this drug on various cell cycle stages might be different, thus inducing different modes of death (apoptotic or mitotic death). the aim of this investigation was to study the effects of bleomycin on human peripheral blood lymphocytes at various cell cycle stages by two different end points (induct...

Objective(s)The aim of this study was to investigate the radioprotective effect of green tea against genotoxicity induced by gamma irradiation in cultured blood lymphocytes from 5 human volunteers.Materials and MethodsPeripheral blood samples were collected from volunteers before and 1, 3 and 5 hr after drinking a decoction 4 g green tea in 280 ml boiling water for 5 constitutive days with the ...