نتایج جستجو برای: human blastocysts

تعداد نتایج: 1645537  

Journal: :JPMA. The Journal of the Pakistan Medical Association 2013
Saki Ghasem Karimian Negar

OBJECTIVE To compare the effect of using open and closed carriers on count of inner cell mass in vitrified mouse blastocyst after warming. METHODS The experimental study was conducted at Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, from April to September 2010. Forty female NMRI (Naval Medical Research Institute, USA) mice were injected with pregnant mare's serum gonadotropi...

2014
Hiroaki Okae Hatsune Chiba Hitoshi Hiura Hirotaka Hamada Akiko Sato Takafumi Utsunomiya Hiroyuki Kikuchi Hiroaki Yoshida Atsushi Tanaka Mikita Suyama Takahiro Arima

DNA methylation is globally reprogrammed during mammalian preimplantation development, which is critical for normal development. Recent reduced representation bisulfite sequencing (RRBS) studies suggest that the methylome dynamics are essentially conserved between human and mouse early embryos. RRBS is known to cover 5-10% of all genomic CpGs, favoring those contained within CpG-rich regions. T...

2018
Yi-Fan Gu Qin-Wei Zhou Shuo-Ping Zhang Chang-Fu Lu Fei Gong Yue-Qiu Tan Guang-Xiu Lu Ge Lin

BACKGROUND The use of assisted reproductive technology (ART) has been reported to increase the incidence of monozygotic twinning (MZT) compared with the incidence following natural conception. It has been hypothesized that splitting of the inner cell mass (ICM) through a small zona hole may result in MZT. In this study, using a cohort of patients undergoing preimplantation genetic diagnosis/scr...

2014
Shu-Tao Qi Li-Feng Liang Ye-Xing Xian Jian-Qiao Liu Weihua Wang

BACKGROUND Aneuploidy is one of the major factors that result in low efficiency in human infertility treatment by in vitro fertilization (IVF). The development of DNA microarray technology allows for aneuploidy screening by analyzing all 23 pairs of chromosomes in human embryos. All chromosome screening for aneuploidy is more accurate than partial chromosome screening, as errors can occur in an...

Journal: :Journal of Assisted Reproduction and Genetics 2021

Journal: :Journal of embryology and experimental morphology 1974
Y C Hsu J Baskar L C Stevens J E Rash

About 1-3 % of mouse blastocysts, which had initially been cultured from the two-cell stage in chemically defined medium or about 3-5 % of blastocysts which were explanted from the uterus, developed to the early somite stage when cultured in vitro on collagen. Twocell eggs were initially cultivated in chemically defined medium to the blastocyst stage. Blastocysts were then transferred to Eagle'...

Hassanpoor S Karimyshoar M Mazochiyan H Roshangar L, Soleimani Rad J

Background: In spite of the great advances in vitrification, the mechanism of embryo damage which occure in some cases and varies from one case to another case is not exactly understood. Since Zona pellucida has an important role on early embryonic development, the aim of the present study is to investigate the effect of vitrification on integrity of zona pellucida and surveillance of blastocys...

Journal: :Human reproduction 2005
Q F Lyu L Q Wu Y P Li Q Pan D E Liu K Xia D S Liang F Cai Z G Long H P Dai J H Xia

BACKGROUND Varied clinical outcomes of assisted hatching (AH) have been reported. We attempt to investigate whether the size of the zona opening created by AH is adequate for blastocyst hatching, and, if not, set up a new method to improve it. METHODS A new AH technique, long zona dissection (LZD), was established, and experiments were performed to compare the effects of different sizes of zo...

Ashraf Moini, Farid Abolhassani, Farnoosh Jafarpour, Khadijeh Karbalaie, Marzieh Derakhshan-Horeh, Mohammad Hossein Nasr-Esfahani*, Sayyed Morteza Hosseini, Somayyeh Ostadhosseini,

Backgrund: Imprinted genes are a unique subset of few genes, which have been differentially methylated region (DMR) in a parental origin-dependent manner during gametogenesis, and these genes are highly protected during pre-implantation epigenetic reprogramming. Several studies heve shown that the particular vulnerability of imprinting genes during suboptimal pre- and peri-conception microenvir...

Journal: :Human reproduction 1997
C Y Fong A Bongso S C Ng C Anandakumar A Trounson S Ratnam

Recent reports suggest that transfer of day 5 blastocysts improves implantation rates in in-vitro fertilization programmes. This paper reports a successful ongoing pregnancy after the transfer of zona-free day 6 expanded and hatching blastocysts. The patient was 37 years old and had undergone six stimulated and two thaw cycles previously, all of which had failed. Stimulation was by down-regulat...

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