BACKGROUND Previous studies using viral cultures rarely reported herpes simplex virus type 2 (HSV-2) isolation from the mouth. We sought to characterize oral HSV-2 shedding as detected by HSV DNA polymerase chain reaction among HSV-2-seropositive men. METHODS Participants collected daily swabs from oral and anogenital areas for HSV detection with a quantitative polymerase chain reaction assay...

Background: Herpes simplex virus type 2 (HSV-2) is a common infection in human immunodeficiency virus (HIV) patients and may accelerate HIV progression by rising HIV viral load and decreasing CD4 count. However, the available data regarding the influence of HSV-2 seropositivity on HIV progression in HIV individuals are inconclusive. Therefore, we aimed to determine HSV-2 seroprevalence in na&iu...

BACKGROUND Despite increasing reports of herpes simplex virus (HSV) type 1 (HSV-1)-associated anogenital herpes, there are very limited data comparing the seroepidemiological profile of and risk factors for HSV-1 and HSV type 2 (HSV-2) infection. METHODS Sexual behaviors were examined as risk factors for prevalent and incident HSV-1 and HSV-2 infections in a community-based cohort of 1,427 HI...

A 131,000 molecular weight herpes simplex virus type 1 (HSV-1) glycoprotein designated antigen number 6 (Ag-6) was previously shown to possess almost exclusively HSV-1-specific antigenic sites. Fused rocket and crossed immunoelectrophoresis of fractions obtained from hydroxylapatite chromatography of crude HSV-1 antigen (Triton X-100-solubilized, infected tissue culture cells) showed that a sub...

Dendritic cells (DC) are critical for stimulation of naive T cells. Little is known about the effect of herpes simplex virus type 2 (HSV-2) infection on DC structure or function or if the observed effects of HSV-1 on human DC are reproduced in murine DC. Here, we demonstrate that by 12 h postinfection, wild-type (wt) HSV-2 (186) abortively infected murine bone marrow-derived DC and induced earl...

background and aims: sexually transmitted infections (stis) are of major concern to clinicians and researchers in the field of reproductive medicine. many sti pathogens cause incurable and often fatal diseases, and have been transmitted through insemination procedures. the role of herpes simplex virus in male infertility has been investigated using the sensitive methods. the aim of this study w...

Herpes simplex virus (HSV) causes a wide range of pathologies in the human central nervous system (CNS) such as stroke. It was proposed that there is an association between HSV infection and stroke with different results reported by the researchers using different methods. This study aimed to investigate the frequency of HSV among stroke patient and control groups. We detected HSV in 28 stroke ...

herpes simplex virus type 1 establishes a latent infection in the peripheral nervous system following primary infection. during latent infection, virus genome exhibit limited transcription, with the hsv lats consistently detected in latency infected ganaglia. following ocular infection viral latency develops in the trigeminal ganglia. in this study pcr has been used for detection of hsv-1 nucli...

purposes: to report a case of primary graft failure (pgf) and systemic manifestations of herpes simplex virus (hsv) infection following transplantation of an hsv-contaminated donor cornea. patient and findings: a 28-year-old male with history of penetrating keratoplasty 12 days before, developed intraocular inflammation with marked anterior chamber reaction, graft edema and elevated intraocular...

BACKGROUND The thymidine kinase (tk) mutagenesis assay is often utilized to determine the frequency of herpes simplex virus (HSV) replication-mediated mutations. Using this assay, clinical and laboratory HSV-2 isolates were shown to have a 10- to 80-fold higher frequency of spontaneous mutations compared to HSV-1. METHODS A panel of HSV-1 and HSV-2, along with polymerase-recombinant viruses e...