One of the major challenges in developing programs for kidney transplantation is represented by presence antibodies targeting HLA donor recipients and, particular cases, incompatibility ABO blood groups among and recipient living donors [...]

The development of anti-factor VIII antibodies is a major complication of the treatment of patients with hemophilia A. Generation of high affinity anti-factor VIII antibodies is dependent on help provided by CD4+ T cells that recognize factor VIII-derived peptides presented on class II major histocompatibility complex on the surface of antigen-presenting cells. In order to identify the immune-d...

Humoral sensitization against immunogenic amino acid (aa) triplets expressed on a rejected graft was analyzed in 83 retransplant candidates. All patients had lost a graft with HLA-A,-B mismatches. The alloantibodies were detected by a complement-dependent cytotoxicity (CDC) technique and an ELISA method in parallel; they were classified as HLA graft-specific (GS) and non-GS antibodies. The aa t...

BACKGROUND HLAMatchmaker is a program to analyze the epitope specificities of HLA antibodies. It considers each HLA allele as a string of eplets. Intralocus and interlocus comparisons between donor and recipient alleles offer a structural assessment of compatibility and an analysis of allele panel reactivity patterns can generate information about epitope specificities of HLA antibodies. Howeve...

In addition to ultrasonographic monitoring of folliculogenesis, urinary luteinizing hormone testing, luteal phase endometrial biopsy, serum antiphospholipid antibodies, etc., immunologic tests are recently being used to evaluate reproductive disorders. In this study, sharing of HLA-A, B, C and DR antigens and the presence of antipaternal cytotoxic antibody (APCA) was assessed using microcyt...

Recent reports have suggested an association of antiDP antibodies with allograft rejection and graft loss, in both primary kidney transplant recipients and in re-grafts [1–3]. However, in most cases, it is difficult to separate the effects of anti-HLA-DP antibodies from other anti-HLA class I and class II antibodies. Among non-conserved haplotypes, HLA-DP allele mismatches occur despite matchin...

BACKGROUND Recent studies have suggested that the birth order effect in allergy may be established during the prenatal period and that the protective effect may originate in the mother. HLA class II disparity between mother and foetus has been associated with significantly increased Th1 production. In this study, we investigated whether production of HLA antibodies 4 years after pregnancy with ...

Multitransfused recipients of random donor platelet concentrates frequently form broadly-reactive human leukocyte antigen (HLA) antibodies which restrict the pool of suitable donors to those who are HLA matched with the recipient. In an effort to expand the donor pool for such patients, a retrospective analysis of the antibodies formed by alloimmunized recipients was performed taking sensitizat...

The development of transplant atherosclerosis, a manifestation of chronic rejection, is the major obstacle to long-term survival of cardiac and renal allografts. The incidence of transplant atherosclerosis is increased in transplant recipients producing antidonor HLA antibodies following transplantation, suggesting that anti-HLA antibodies play a role in the pathogenesis of the disease. We have...

This review focuses on reports on fetal/neonatal alloimmune thrombocytopenia (FNAITP) – presumably due to alloimmunization to HLA antigens – published during the last 6 years. Platelets express HLA class I antigens, and HLA antibodies are known to adversely affect the success of platelet transfusions. Likewise, it is conceivable that HLA antigens can serve as targets leading to HLA antibody-ind...