A novel 2',4'-BNA/LNA analog bridged by guanidine, termed as guanidine bridged nucleic acid (GuNA), was synthesized and incorporated into oligonucleotides. Thermal stabilities and nuclease resistance of GuNA-modified oligonucleotides were investigated and compared with those of 2',4'-BNA/LNA and natural DNA oligonucleotides. GuNA exhibited interestingly high binding affinity towards complementa...

A simple and Noval Synthesis of guanidine derivatives is presented in this study. The construction of the Central basic Scaffolds is achieved by the using of HgCl2 as a promoter. The Formation of product of substituted guanidines controlled by amount of reactant. Further a new fast-tract access to N-1(Boc)-N-(2) cyclohexyl-N(3) phenyl-guanidine was developed.

Guanidine and its alkyl analogs stimulate the neuromuscular junction presynaptically by inhibiting voltage-gated potassium (Kv) channels, leading to enhanced release of acetylcholine in the synaptic cleft. This stimulatory effect of guanidine underlies its use in the therapy for the neuromuscular diseases myasthenic syndrome of Lambert-Eaton and botulism. The therapeutic use of guanidine is lim...

Staphylococcal enterotoxin types A and C, were observed by viscosimetry and near-ultraviolet difference spectroscopy to unfold at concentrations of aqueous guanidine hydrochloride greater than 1 M. Apparent rate constants of unfolding calculated from spectral curves differed markedly for the two enterotoxins. Rate constants for the unfolding of enterotoxin A in 2 or 3 M guanidine hydrochloride ...

The mechanism of guanidine-catalyzed enantioselective isomerization of 3-alkynoates to allenoates is investigated using density functional theory methods. The calculations predict that the isomerization reaction includes two hydrogen-transfer steps and one conformational change mediated by the TBO catalyst. The first hydrogen-transfer step corresponds to the migration of hydrogen from C(4) of t...

Infection of cells treated with guanidine and actinomycin D and then washed to remove the guanidine inhibition of virus growth had no effect on antiviral activity already established by interferon. Protein synthesis in interferon-treated cells infected under these conditions was decreased as compared to control cells similarly treated but not exposed to interferon. In these control cells, analy...

A set of small nonpeptidic diaryl phosphonate inhibitors was prepared. The phosphonate derivatives were synthesized through Birum-Oleksyszyn reaction followed by reduction of the nitro-group which undergo to Boc-protected guanidine derivatives. After removing of Boc-group by TFAA/DCM afford the guanidine phosphonates.

Polycyclic guanidine natural products, such as batzelladines A, E, and F, exhibit a rich and diverse array of interesting biological activities (Figure 1). Some polycyclic guanidine alkaloids have been shown to inhibit protein–protein interactions, including the binding of HIV gp120 to CD4 on human T-cells. Furthermore, many polycyclic guanidines display potent antiviral, antimalarial, and immu...

We have carefully examined the frequency of guanidine-resistant revertants in six different clonal pools of guanidine-dependent mutants of type 1 poliovirus. The mutation frequency was (6.5 +/- 6.3) x 10(-4) (with all amino acid substitutions occurring at position 227). The minimal corrected base substitution frequency per single nucleotide site in the codon for amino acid 227 was (2.1 +/- 1.9)...

cDNA fragments representing the region in polypeptide 2C containing mutations in a guanidine-resistant or -dependent mutant were cloned into the wild-type background of an infectious clone. Transfection of COS-1 cells with these plasmids yielded viruses that were either completely resistant to 2.0 mM guanidine hydrochloride or dependent on this concentration of drug for growth.