objective: the aim of this study was to construct a pcdna3.1+ vector containing fmdv type o/irn/1/2007-vp1 gene, protein expression in bhkt7 cells and evaluation of immune response in balb/c mice. materials and methods: fmdv type o/irn/1/2007 was isolated from a cattle in ray in 2007 and serotyped. the purified vp1 gene was sub-cloned into the ptz57r/t vector and pcdna3.1+ expression vector. t...

Proteins of the green fluorescent protein (GFP) family are well known owing to their unique biochemistry and extensive use as in vivo markers. We discovered that GFPs of diverse origins can act as light-induced electron donors in photochemical reactions with various electron acceptors, including biologically relevant ones. Moreover, via green-to-red GFP photoconversion, this process can be obse...

Many models of psychopathology include a single general factor (GFP) or “ p factor” to account for covariation across symptoms. The Adolescent Brain Cognitive Development (ABCD) Study provides rich opportunity study the development GFP. However, variety approaches modeling GFP have emerged, raising questions about how choices affect estimated scores. We used ABCD baseline assessment (ages 9–10 ...

Nakano first discovered the “Mitochondrial cells” (Mito Cells), which can survive for long term despite lacking a nucleus. However, there was no clear evidence to indicate whether MitoCells were alive. To resolve this question, we constructed GFP-actin induced Mito Cell (GFP-actin Cell). Herein, report molecular analysis of both and MitoCells. Our results suggest that have minimum amounts prote...