Systemic sclerosis (SSc) is characterized by excessive deposition of extracellular matrix (ECM) in the involved organs. Fibroblasts are activated and develop an uncontrolled synthesis collagen other ECM constituents. Previous studies have focused on cultured fibroblasts, but interpretation results been limited due to possible selection fibroblast populations. To better understand heterogeneity ...

Mesenchymal stem/stromal cells (MSCs) and fibroblasts are present in normal tissues to support tissue homeostasis. Both share common pathways have a number of features, such as spindle-shaped morphology, connective localization, multipotency. In inflammation, nonspecific response injury, MSC the main players. Two mechanisms their mode action been defined: immunomodulation regeneration. Followin...

Little is known about miRNA decay, especially in skin wound healing. Here, by small RNA sequencing human and tissues, we identified 12 miRNAs that 5P:3P strand ratios significantly changed. To rule out the effects caused cellular composition, performed CD45- epidermal cells (mainly keratinocytes) CD90+ dermal (fibroblasts) isolated from wounds. Our results showed ratio of miR-X changed both cel...

Photoaging mainly occurs due to ultraviolet (UV) radiation, and is accompanied by increased secretion of matrix metalloproteinases (MMPs) degradation collagen. UV radiation induces cell senescence in the skin; however, role senescent cells photoaging remains unclear. Therefore, elucidate photoaging, we evaluated effect senolytics a mouse model investigated underlying mechanism their antiaging e...

NRF2 is a master regulator of the antioxidative response that was recently proposed as potential extracellular matrix (ECM) gene expression. Fibroblasts are major ECM producers in all connective tissues, including dermis. A better understanding NRF2-mediated regulation skin fibroblasts thus great interest for homeostasis maintenance and aging protection. In this study, we investigate impact dow...

Wound healing is a highly dynamic process over spatial and temporal scales. Key to wound outcomes are fibroblasts, whose precise functions in tissue repair only partially understood. We constructed an atlas of mouse skin using several published single-cell RNA-seq (scRNA-seq) datasets. Using scRNA-seq data integration across time, we identified key cell types states that both shared as well uni...