نتایج جستجو برای: expression optimization
تعداد نتایج: 1184372 فیلتر نتایج به سال:
A novel strategy to increase protein expression yield is unintended induction of expression in complex media, called auto-induction. This method can be used to express proteins under control of the lac promoter without any need to monitor bacterial growth pattern, and addition of specific expression inducers such as Isopropyl β-D-1-thiogalactopyranoside (IPTG) at proper time. In the present stu...
................................................................................................................................................ 1 1 BACKGROUND ...................................................................................................................................... 3 1.1 INTRODUCTION ....................................................................
conclusions a fusion tag from the pet32 expression plasmid permits the recovery of the recombinant fusion fgf-2 from e. coli, without affecting its biological activity. objectives the objective of this study was to overexpress and purify the high-level active human bfgf in escherichia coli (e. coli). materials and methods this experimental study was conducted in the islamic republic of iran. af...
مسئله ی یافتن کلیک بیشینه گراف maximum clique problem (mcp)، از جمله مسائل np-complete است که به یافتن بزرگترین زیرگراف کامل در یک گراف ساده اشاره دارد و در موارد متنوعی از جمله نظریه کدگذاری، هندسه و شبکه های اجتماعی کاربرد دارد. در این پژوهش الگوریتمی ترکیبی برای حل مسئله ی کلیک بیشینه گراف پیشنهاد شده است. این الگوریتم ترکیبی از یک روش حریصانه ابتکاری و الگوریتم های مبتنی بر هوش جمعی بهینه س...
one of the most important number sequences in mathematics is fibonacci sequence. fibonacci sequence except for mathematics is applied to other branches of science such as physics and arts. in fact, between anesthetics and this sequence there exists a wonderful relation. fibonacci sequence has an importance characteristic which is the golden number. in this thesis, the golden number is observed ...
Activin A is a member of the transforming growth factor β super family. Because of its extensive clinical usages, its recombinant production is beneficial. In this study, activin A was expressed in E. coli using the pET 21a expression vector. The optimization of the activin A production in E. coli was done by using the response surface methodology (RSM). At this stage, the effect of IPTG and la...
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