OBJECTIVES Enzymes are commonly used in the baking industry, as they can improve dough quality and texture and lengthen the shelf life of the final product. There is little published information highlighting exposure to enzymes (other than fungal alpha-amylase) in the baking industry, therefore the purpose of this study was to identify antibodies and develop assays for the measurement of a vari...

Partially digested gluten fragments from grains including wheat, rye, spelt and barley are responsible for triggering an inflammatory response in the intestinal tract of Celiac Disease (CD) Non-Celiac Gluten Sensitive (NCGS) individuals. Fermentation is effective method to metabolize gluten, with enzymes bacterial or fungal species being released help this process. However, levels commercially ...

DOI: 10.1002/jssc.202100066 The cover picture shows a schematic diagram of liquid chromatography with tandem mass spectrometry method for analyzing microsomal and soluble epoxide hydrolase in rat heart, kidney, liver S9 samples. Protein quantification was realized through targeted proteomics the determination trypsin-specific surrogate peptides after digestion. A multiple reaction monitoring po...

Oxidative stress (OS)-induced mitochondrial damage is a risk factor for primary open-angle glaucoma (POAG). Mitochondria-targeted novel antioxidant therapies could unearth promising drug candidates the management of POAG. Previously, our dual-acting hybrid molecule SA-2 with nitric oxide-donating and activity reduced intraocular pressure improved aqueous humor outflow in rodent eyes. Here, we e...

The anti-diabetic potential of whole unripe jackfruit (peel with pulp, flake, and seed) was investigated using inhibitory assays for α-glucosidase, α-amylase, aldose reductase, glycation at multiple stages. Using activity-guided repeated fractionation on a silica gel column chromatography, dietary flavonoid rutin potent antihyperglycemic activity extracted from the methanol extract flour (MJ). ...

background: reactive oxygen species (ros)-induced lipidperoxidation can lead to dysfunction of sperm and thereby, infertility may be occurred. so, always there is a balance between amount of ros and anti-oxidant molecules in semen. anti-oxidant enzymes of sperm; superoxide dismutase (sod), glutathione peroxidase (gpx), catalse and zinc and selenium can protect it from destructive effects of ros...

Background and objective: Diabetes is the most common cause of chronic renal disease globally. Diabetic nephropathy (DN) one serious consequences type 2 diabetes. Melatonin, a powerful antioxidant that has been shown to alleviate DN, deficiency functional relationship between melatonin insulin have linked etiology diabetes mellitus. The purpose this research assess level enzyme activity (catala...

We describe a method for the detection of isoforms of several glycolytic enzymes by activity staining after native PAGE. The staining is based on coupled enzyme assays carried out on the gel after electrophoresis and is linked to the disappearance of NADH, which is visualized by fluorescence. This method offers reliable and sensitive detection for phosphoenolpyruvate carboxylase, PPi-dependent ...

Cellulose, the major constituent of all plant materials and the most abundant organic molecule on the Earth, is a linear biopolymer of glucose molecules, connected by β-1,4-glycosidic bonds. Enzymatic hydrolysis of cellulose requires mixtures of hydrolytic enzymes including endoglucanases, exoglucanases (cellobiohydrolases), and β-glucosidases acting in a synergistic manner. In biopolymer hydro...

Deoxynivalenol (DON) commonly infects agricultural foods; it exhibits toxicity by inducing oxidative stress and inhibiting protein synthesis. Nuclear factor erythroid 2-related 2 (NRF2) regulates the cellular antioxidant response. We investigated cytotoxicity of DON its effect on NRF2 response in HepG2 cells. The Methyl Thiazol Tetrazolium (MTT), glutathione (GSH) ATP assays evaluated toxicity,...